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. 2017 Apr 20;18(4):875.
doi: 10.3390/ijms18040875.

Aneurysm-Specific miR-221 and miR-146a Participates in Human Thoracic and Abdominal Aortic Aneurysms

Premakumari Venkatesh  1 , Julie Phillippi  2 , Sasanka Chukkapalli  3 , Mercedes Rivera-Kweh  4 , Irina Velsko  5 , Thomas Gleason  6 , Paul VanRyzin  7 , Seyed Hossein Aalaei-Andabili  8 , Ravi Kiran Ghanta  9 , Thomas Beaver  10 , Edward Kar Leung Chan  11 , Lakshmyya Kesavalu  12   13
Affiliations

Aneurysm-Specific miR-221 and miR-146a Participates in Human Thoracic and Abdominal Aortic Aneurysms

Premakumari Venkatesh et al. Int J Mol Sci. .

Abstract

Altered microRNA expression is implicated in cardiovascular diseases. Our objective was to determine microRNA signatures in thoracic aortic aneurysms (TAAs) and abdominal aortic aneurysms (AAAs) compared with control non-aneurysmal aortic specimens. We evaluated the expression of fifteen selected microRNA in human TAA and AAA operative specimens compared to controls. We observed significant upregulation of miR-221 and downregulation of miR-1 and -133 in TAA specimens. In contrast, upregulation of miR-146a and downregulation of miR-145 and -331-3p were found only for AAA specimens. Upregulation of miR-126 and -486-5p and downregulation of miR-30c-2*, -155, and -204 were observed in specimens of TAAs and AAAs. The data reveal microRNA expression signatures unique to aneurysm location and common to both thoracic and abdominal pathologies. Thus, changes in miR-1, -29a, -133a, and -221 are involved in TAAs and miR-145, -146, and -331-3p impact AAAs. This work validates prior studies on microRNA expression in aneurysmal diseases.

Keywords: abdominal aortic aneurysm; microRNA; thoracic aortic aneurysm.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Alterations in microRNA profiles of miR-1 (a), miR-21 (b), miR-29a (c), miR-30c-2* (d), miR-124 (e), and mir-126 (f) by qRT-PCR. Expression levels in TAA (n = 11) and AAA (n = 3) as compared with non-aneurysmal control aorta (n = 8). The relative amount of each miRNA was normalized to small nucleolar RNA (RNU44). Statistically significant differences: * p < 0.05, ** p < 0.01, *** p < 0.001.
Figure 2
Figure 2
Alteration in microRNA profiles of miR-133a (a), miR-145 (b), miR-146a (c), miR-155 (d), miR-204 (e), and miR-221 (f) by qRT-PCR. Expression levels in TAA (n = 11) and AAA (n = 3) as compared with non-aneurysmal control aorta (n = 8). The relative amount of each miRNA was normalized to RNU44. Statistically significant differences: * p < 0.05, ** p < 0.01.
Figure 3
Figure 3
Alteration in microRNA profiles of miR-222 (a), miR-331-3p (b), and miR-486-5p (c) by qRT-PCR. Expression levels in TAA (n = 11) and AAA (n = 3) as compared with non-aneurysmal control aorta (n = 8). The relative amount of each miRNA was normalized to RNU44. Statistically significant differences: * p < 0.05.
Figure 4
Figure 4
Heat map (Human miRNA microarray) depicting 45 differentially expressed miRNAs in TAA. Differentially expressed miRNAs (p < 0.05 and absolute value (fold change) > 1.5) in TAA specimens as compared with non-aneurysmal control aorta is shown. Arrows indicate the miRNAs whose expression was similar in qRT-PCR analysis. TAA (n = 5) and normal (n = 3).
Figure 5
Figure 5
Volcano map demonstrating differences in miRNA expression levels in TAA by microarray. The log-fold change is plotted against the log odds of differential expression. The miRNAs with significant differences in expression levels between the TAA (n = 5) and control (n = 3) groups (p < 0.05) after Benjamini-Hochberg correction are indicated.
Figure 6
Figure 6
Dominant cardiovascular miRNAs patterns observed in TAA and AAA.
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