Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Apr 20;12(4):e0175941.
doi: 10.1371/journal.pone.0175941. eCollection 2017.

The culturable mycobiota of Flabellia petiolata: First survey of marine fungi associated to a Mediterranean green alga

Giorgio Gnavi  1 , Laura Garzoli  1 , Anna Poli  1 , Valeria Prigione  1 , Gaëtan Burgaud  2 , Giovanna Cristina Varese  1
Affiliations

The culturable mycobiota of Flabellia petiolata: First survey of marine fungi associated to a Mediterranean green alga

Giorgio Gnavi et al. PLoS One. .

Abstract

Algae-inhabiting marine fungi represent a taxonomically and ecologically interesting group of microorganisms still largely neglected, especially in temperate regions. The aim of this study was to isolate and to identify the culturable mycobiota associated with Flabellia petiolata, a green alga frequently retrieved in the Mediterranean basin. Twenty algal thalli were collected from two different sampling sites in the Mediterranean Sea (Elba Island, Italy). A polyphasic approach showed the presence of a relevant alga-associated mycobiota with 64 taxa identified. The fungal isolates belonged mainly to Ascomycota (61 taxa), while only three Basidiomycota were detected. The phylogenetic position of sterile mycelia and cryptic taxa, inferred on the basis of LSU partial region, highlighted the presence of putative new phylogenetic lineages within Dothideomycetes and Sordariomycetes. This work represents the first quali-quantitative analysis of the culturable mycobiota associated to a green alga in the Mediterranean Sea.

PubMed Disclaimer

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Sampling sites.
Elba Island (Livorno), Tuscany, Tyrrhenian Sea (Mediterranean Sea) Italy.
Fig 2
Fig 2. Bayesian phylogram of Pleosporales (Dothideomycetes) based on rDNA large subunit (LSU).
Branch numbers indicate BPP over 0.70; ML bootstrap > 50%. Thirteen fungal isolates (indicated as MUT) are included. Strains from marine sources are labelled with symbol Ψ. Bar = expected changes per site (0.03).
Fig 3
Fig 3. Bayesian phylogram of Capnodiales (Dothideomycetes) based on rDNA large subunit (LSU).
Branch numbers indicate BPP over 0.70; ML bootstrap > 50%. Five fungal isolates (indicated as MUT) are included. Strains from marine sources are labelled with symbol Ψ. Bar = expected changes per site (0.05).
Fig 4
Fig 4. Bayesian phylogram of Leotiomycestes based on rDNA large subunit (LSU).
Branch numbers indicate BPP over 0.70; ML bootstrap > 50%. Two fungal isolates (indicated as MUT) are included. Strains from marine sources are labelled with symbol Ψ. Bar = expected changes per site; value (0.04).
Fig 5
Fig 5. Bayesian phylogram of Sordariomycestes based on rDNA large subunit (LSU).
Branch numbers indicate BPP over 0.70; ML bootstrap > 50%. Thirteen fungal isolates (indicated as MUT) are included. Strains from marine sources are labelled with symbol Ψ. Bar = expected changes per site (0.07).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Richards TA, Jones MD, Leonard G, Bass D, Marine fungi: their ecology and molecular diversity. Ann Rev Mar Sci 2012;4:495–522. 10.1146/annurev-marine-120710-100802 - DOI - PubMed
    1. Jones EBG, Pang K-L, Marine fungi and fungal-like organisms Marine and Freshwater Botany. Walter de Gruyter Berlin-Boston; 2012.
    1. Jones EBG, Are there more marine fungi to be described? Bot March 2011;54:343–54.
    1. Jones EG, Suetrong S, Sakayaroj J, Bahkali AH, Abdel-Wahab MA, Boekhout T, et al. Classification of marine Ascomycota, Basidiomycota, Blastocladiomycota and Chytridiomycota. Fungal Divers 2015;73:1–72.
    1. Manohar CS, Raghukumar C, Fungal diversity from various marine habitats deduced through culture-independent studies. Fems Microbiol Lett 2013; 341:69–78. 10.1111/1574-6968.12087 - DOI - PubMed