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. 1988 Sep;85(18):6778-82.
doi: 10.1073/pnas.85.18.6778.

Galactose at the nonreducing terminus of O-linked oligosaccharides of mouse egg zona pellucida glycoprotein ZP3 is essential for the glycoprotein's sperm receptor activity

J D Bleil  1 , P M Wassarman
Affiliations

Galactose at the nonreducing terminus of O-linked oligosaccharides of mouse egg zona pellucida glycoprotein ZP3 is essential for the glycoprotein's sperm receptor activity

J D Bleil et al. Proc Natl Acad Sci U S A. 1988 Sep.

Erratum in

  • Proc Natl Acad Sci U S A 1988 Dec;85(24):9600

Abstract

During fertilization in mice, zona pellucida glycoprotein ZP3 mediates initial sperm-egg interactions by serving as receptor for sperm. Purified egg ZP3, as well as ZP3-derived O-linked oligosaccharides, exhibit sperm receptor activity in vitro. We report that treatment of purified egg ZP3 and ZP3-derived O-linked oligosaccharides with either alpha-galactosidase or galactose oxidase results in loss of sperm receptor activity. In the latter case, sperm receptor activity can be restored to the oxidized glycoprotein and O-linked oligosaccharides by treatment with sodium borohydride. We conclude that galactose, located in alpha-linkage at the nonreducing terminus of O-linked oligosaccharides, is at least one of the sugar determinants on ZP3 responsible for binding of sperm to the zona pellucida.

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References

    1. EMBO J. 1983;2(9):1451-6 - PubMed
    1. Annu Rev Cell Biol. 1987;3:109-42 - PubMed
    1. Nature. 1973 Jan 26;241(5387):256-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2456-60 - PubMed
    1. Cell. 1980 Jul;20(3):873-82 - PubMed

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