Multi-photon microscopy in cardiovascular research

Zhuojun Wu¹, Timo Rademakers², Fabian Kiessling³, Michael Vogt⁴, Erik Westein⁵, Christian Weber⁶, Remco T A Megens⁶, Marc van Zandvoort⁷

Affiliations

¹ Institute for Molecular Cardiovascular Research, University Clinic, RWTH-Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany; Institute for Experimental Molecular Imaging, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
² Department of Molecular Cell Biology, School for Cardiovascular Diseases (CARIM), Maastricht University, Universiteitssingel 50, 67229 ER Maastricht, The Netherlands; Department of Pathology, Maastricht University Medical Centre, P. Debyelaan 25, 6229 HX Maastricht, The Netherlands.
³ Institute for Experimental Molecular Imaging, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
⁴ Institute of Laboratory Animal Science and Experimental Surgery, University Clinic, RWTH-Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
⁵ Atherothrombosis and Vascular Biology, Baker IDI Heart and Diabetes Institute, 75 Commercial Rd, Melbourne 3004, Australia.
⁶ Institute of Cardiovascular Prevention, Ludwig Maximillian's University, Pettenkoferstrasse 8a, 80336 Munich, Germany; School for Cardiovascular Diseases (CARIM), Maastricht University, Universiteitssingel 50, 67229 ER Maastricht, The Netherlands.
⁷ Institute for Molecular Cardiovascular Research, University Clinic, RWTH-Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany; Department of Molecular Cell Biology, School for Cardiovascular Diseases (CARIM), Maastricht University, Universiteitssingel 50, 67229 ER Maastricht, The Netherlands. Electronic address: mamj.vanzandvoort@maastrichtuniversity.nl.

PMID: 28434997
DOI: 10.1016/j.ymeth.2017.04.013

Review

Multi-photon microscopy in cardiovascular research

Zhuojun Wu et al. Methods. 2017.

. 2017 Nov 1:130:79-89.

doi: 10.1016/j.ymeth.2017.04.013. Epub 2017 Apr 18.

Authors

Zhuojun Wu¹, Timo Rademakers², Fabian Kiessling³, Michael Vogt⁴, Erik Westein⁵, Christian Weber⁶, Remco T A Megens⁶, Marc van Zandvoort⁷

Affiliations

¹ Institute for Molecular Cardiovascular Research, University Clinic, RWTH-Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany; Institute for Experimental Molecular Imaging, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
² Department of Molecular Cell Biology, School for Cardiovascular Diseases (CARIM), Maastricht University, Universiteitssingel 50, 67229 ER Maastricht, The Netherlands; Department of Pathology, Maastricht University Medical Centre, P. Debyelaan 25, 6229 HX Maastricht, The Netherlands.
³ Institute for Experimental Molecular Imaging, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
⁴ Institute of Laboratory Animal Science and Experimental Surgery, University Clinic, RWTH-Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
⁵ Atherothrombosis and Vascular Biology, Baker IDI Heart and Diabetes Institute, 75 Commercial Rd, Melbourne 3004, Australia.
⁶ Institute of Cardiovascular Prevention, Ludwig Maximillian's University, Pettenkoferstrasse 8a, 80336 Munich, Germany; School for Cardiovascular Diseases (CARIM), Maastricht University, Universiteitssingel 50, 67229 ER Maastricht, The Netherlands.
⁷ Institute for Molecular Cardiovascular Research, University Clinic, RWTH-Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany; Department of Molecular Cell Biology, School for Cardiovascular Diseases (CARIM), Maastricht University, Universiteitssingel 50, 67229 ER Maastricht, The Netherlands. Electronic address: mamj.vanzandvoort@maastrichtuniversity.nl.

PMID: 28434997
DOI: 10.1016/j.ymeth.2017.04.013

Abstract

Multiphoton laser scanning microscopy has proven profound value for ex vivo 3D histology and in vivo imaging of motionless tissue. The development of triggering systems and fast imaging methods, combined with advanced preparation procedures solved the challenging task of intravital imaging of the fast pulsating heart and major arteries in animals and further increased the popularity of intravital multiphoton imaging in cardiovascular research. This review article will highlight the potential of multiphoton microscopy for the visualization and characterization of dynamical and structural processes involved in cardiac and vascular diseases, both in an ex vivo and an intravital animal setting. Examples will be given how multiphoton microscopy can be applied to imaging of atherosclerotic plaque development and progression at subcellular level as well as to intravital imaging of inflammatory processes in the heart. In addition to highlighting the potential of multiphoton microscopy in preclinical cardiovascular research, we will discuss how this tool and its applications may be clinically translated to support disease diagnosis and therapy in patients.

Keywords: Cardiovascular imaging; Carotid artery; Intravital imaging; Non-linear microscopy; Optical imaging.

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Multi-photon microscopy in cardiovascular research

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Multi-photon microscopy in cardiovascular research

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