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. 2017 Jun 10:251:94-98.
doi: 10.1016/j.jbiotec.2017.04.015. Epub 2017 Apr 20.

Kinetics of chitin deacetylase activation by the ionic liquid [Bmim][Br]

Izabela Aspras  1 , Malgorzata M Jaworska  2 , Andrzej Górak  3
Affiliations

Kinetics of chitin deacetylase activation by the ionic liquid [Bmim][Br]

Izabela Aspras et al. J Biotechnol. .

Abstract

Chitin deacetylase is the only known enzyme that can deacetylate the N-acetyl-d-glucosamine units in chitin and chitosan to D-glucosamine. Unfortunately, this enzyme, originally obtained from fungi, usually has low activity. Here, we present that it is possible to enhance the activity of chitin deacetylase using the ionic liquid [Bmim][Br]. An increase in activity of up to 160% from the basal chitin deacetylase activity was observed. Kinetic investigations suggest that [Bmim][Br] is a non-essential activator.

Keywords: Chitin deacetylase; Enzyme; Ionic liquids; Non-essential activation; [Bmim][Br].

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