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. 2017 Mar;46(3):351-356.

Utilization of PFGE as a Powerful Discriminative Tool for the Investigation of Genetic Diversity among MRSA Strains

Solmaz Ohadian Moghadam  1   2 , Mohammad Reza Pourmand  2   3 , Masoumeh Douraghi  2 , Samira Sabzi  2 , Parisa Ghaffari  3
Affiliations

Utilization of PFGE as a Powerful Discriminative Tool for the Investigation of Genetic Diversity among MRSA Strains

Solmaz Ohadian Moghadam et al. Iran J Public Health. 2017 Mar.

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant challenge to the burn patient. The implementation of proper monitoring programs and prompt identification of epidemic MRSA strains are critical to consequently control and eradicate potential outbreaks. This study aimed to define the genetic relatedness of MRSA strains isolated from burn patients by analyzing the large fragments of DNA.

Methods: In this cross-sectional study, 126 pus/wound swabs from skin and soft tissue infections (SSTIs) were collected from inpatients of Shahid Motahari Burn Center (Tehran, Iran) in 2013. Then, molecular typing of MRSA was achieved by Pulsed-Field Gel Electrophoresis (PFGE).

Results: The PFGE analysis of MRSA indicated 31 single types and 5 common types. There was a significant diversity in the chromosomal digestion pattern of the MRSA strains explained by the acquisition of MRSA from various sources.

Conclusion: The permanent import of novel types of MRSA strains despite the rigorous infection control measures carried out within the center. The importance of PFGE in understanding the epidemiology of MRSA may serve as a basis for the development of rational control strategies.

Keywords: Burn units; Methicillin-resistant staphylococcus aureus; Molecular typing; Pulsed-field gel electrophoresis.

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Figures

Fig. 1:
Fig. 1:
Dendrogram based on the relationships among MRSA isolates derived from the UPGMA and Dice coefficients, using Gel ComparII software
See this image and copyright information in PMC

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