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. 1979 Jan;76(1):86-90.
doi: 10.1073/pnas.76.1.86.

Competitive inhibition of colchicine binding to tubulin by microtubule-associated proteins

J Nunez, A Fellous, J Francon, A M Lennon

Competitive inhibition of colchicine binding to tubulin by microtubule-associated proteins

J Nunez et al. Proc Natl Acad Sci U S A. 1979 Jan.

Abstract

Microtubule-associated proteins (MAPs) promote tubulin polymerization, whereas colchicine inhibits this process. In this paper, MAPs have been shown to inhibit colchicine binding to tubulin in a competitive manner. Attempts were made to identify which of the MAPs fraction(s) was responsible; both tau protein (a thermostable molecule with a molecular weight of approximately 70,000) and a high molecular weight fraction (HMW) were able to compete with colchicine. In contrast, Mg2+, which also induces microtubule assembly in vitro, had no effect on colchicine binding to tubulin.

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