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. 1979 Feb;76(2):640-4.
doi: 10.1073/pnas.76.2.640.

Human leukocyte interferon: production, purification to homogeneity, and initial characterization

M Rubinstein, S Rubinstein, P C Familletti, R S Miller, A A Waldman, S Pestka

Human leukocyte interferon: production, purification to homogeneity, and initial characterization

M Rubinstein et al. Proc Natl Acad Sci U S A. 1979 Feb.

Abstract

A method of fractionating proteins by high-performance liquid partition chromatography has been developed and used for isolation and purification to homogeneity of one of the species of human leukocyte interferon. The homogeneous interferon exhibited a sharp peak on high-performance liquid chromatography and a single narrow band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol. Extraction of the gel gave a single sharp peak of antiviral activity coinciding with the protein band. The specific activity of pure interferon was found to be 2--4 X 10(8) units/mg, based on amino acid analysis. The molecular weight is 17,500--18,000.

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References

    1. C R Seances Soc Biol Fil. 1958;152(11):1627-9 - PubMed
    1. Proc R Soc Lond B Biol Sci. 1957 Sep 12;147(927):268-73 - PubMed
    1. Proc R Soc Lond B Biol Sci. 1957 Sep 12;147(927):258-67 - PubMed
    1. J Gen Virol. 1976 Nov;33(2):225-36 - PubMed
    1. Tex Rep Biol Med. 1977;35:117-25 - PubMed

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