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. 1979 Feb;76(2):700-4.
doi: 10.1073/pnas.76.2.700.

Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4

D Belin, J Hedgpeth, G B Selzer, R H Epstein

Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4

D Belin et al. Proc Natl Acad Sci U S A. 1979 Feb.

Abstract

We have determined the sequence of a ribosome-protected region of T4 rIIB mRNA labeled in vivo. The rIIB mutant HD263, which is temperature sensitive for translation, has an altered initiation codon (AUA instead of AUG). Because wild-type and HD263 proteins have the same fMet peptide, this AUA is used to initiate translation in the mutant rIIB mRNA. We have also identified a six-base sequence which is complementary to the 3" end of the 16S rRNA.

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References

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