doi: 10.1073/pnas.76.2.700.
Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4
- PMID: 284393
- PMCID: PMC383023
- DOI: 10.1073/pnas.76.2.700
Item in Clipboard
Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4
Proc Natl Acad Sci U S A.
1979 Feb.
Abstract
We have determined the sequence of a ribosome-protected region of T4 rIIB mRNA labeled in vivo. The rIIB mutant HD263, which is temperature sensitive for translation, has an altered initiation codon (AUA instead of AUG). Because wild-type and HD263 proteins have the same fMet peptide, this AUA is used to initiate translation in the mutant rIIB mRNA. We have also identified a six-base sequence which is complementary to the 3" end of the 16S rRNA.
Similar articles
-
Bacteriophage T4 rIIB protein synthesis with a temperature-sensitive mutation in the rIIB initiation codon.Mol Gen Genet. 1979 Mar 9;171(1):35-42. doi: 10.1007/BF00274012. Mol Gen Genet. 1979. PMID: 286150
-
Mutations of bacteriophage T7 that affect initiation of synthesis of the gene 0.3 protein.Proc Natl Acad Sci U S A. 1978 Jun;75(6):2741-5. doi: 10.1073/pnas.75.6.2741. Proc Natl Acad Sci U S A. 1978. PMID: 275843 Free PMC article.
-
Mutations affecting translation of the bacteriophage T4 rIIB gene cloned in Escherichia coli.Mol Gen Genet. 1987 May;207(2-3):224-32. doi: 10.1007/BF00331582. Mol Gen Genet. 1987. PMID: 3112515
-
Recognition of messenger RNA during translational initiation in Escherichia coli.Biochimie. 1984 Jan;66(1):1-29. doi: 10.1016/0300-9084(84)90188-3. Biochimie. 1984. PMID: 6370317 Review.
-
[Site-directed mutagenesis on bacteriophage Qbeta RNA (author's transl)].Tanpakushitsu Kakusan Koso. 1978;23(3):159-69. Tanpakushitsu Kakusan Koso. 1978. PMID: 349607 Review. Japanese. No abstract available.
Cited by
-
Initiation of translation at an AUA codon for an archaebacterial protein gene expressed in E.coli.Nucleic Acids Res. 1991 Oct 11;19(19):5169-72. doi: 10.1093/nar/19.19.5169. Nucleic Acids Res. 1991. PMID: 1923803 Free PMC article.
-
Sequence of a 1.26-kb DNA fragment containing the structural gene for E.coli initiation factor IF3: presence of an AUU initiator codon.EMBO J. 1982;1(3):311-5. doi: 10.1002/j.1460-2075.1982.tb01166.x. EMBO J. 1982. PMID: 6325158 Free PMC article.
-
Transcription initiation at the tet promoter and effect of mutations.Nucleic Acids Res. 1988 Aug 11;16(15):7269-85. doi: 10.1093/nar/16.15.7269. Nucleic Acids Res. 1988. PMID: 3045754 Free PMC article.
-
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.Microbiol Rev. 1983 Mar;47(1):1-45. doi: 10.1128/mr.47.1.1-45.1983. Microbiol Rev. 1983. PMID: 6343825 Free PMC article. Review. No abstract available.
-
Initiator tRNA may recognize more than the initiation codon in mRNA: a model for translational initiation.Proc Natl Acad Sci U S A. 1985 Jul;82(14):4587-91. doi: 10.1073/pnas.82.14.4587. Proc Natl Acad Sci U S A. 1985. PMID: 3860810 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
