Correction: Functional Constraint Profiling of a Viral Protein Reveals Discordance of Evolutionary Conservation and Functionality

Abstract

[This corrects the article DOI: 10.1371/journal.pgen.1005310.].

Fig 2. Fitness profiling of PA influenza virus polymerase subunit.

(A) Correlations of log₁₀ relative frequency of individual point mutations between replicates are shown. Relative frequency_{mutation i} = (Occurrence frequency_{mutation i})/(Occurrence frequency_WT) (B) Log₁₀ RF indices for silent mutations, nonsense mutations, and missense mutations are shown as histograms. Point mutations located at the 5 terminal 400 bp and 3 terminal 400 bp regions are not included in this analysis to avoid complication by the vRNA packaging signal [93, 94]. (C) The locations of the PA C-terminal domain and the PA N-terminal domain are shown as white boxes. The locations of the mutated regions in each mutant library are shown as green boxes. Log₁₀ RF indices for individual point mutations are plotted across the PA gene. Each point mutation is colored coded as in panel B. Purple: silent mutations; Cyan: nonsense mutations; Brown: missense mutations. A smooth curve was fitted by loess and plotted for each point mutation type.