doi: 10.1073/pnas.76.2.977.
Identification of inosine and hypoxanthine as endogenous ligands for the brain benzodiazepine-binding sites
- PMID: 284422
- PMCID: PMC383112
- DOI: 10.1073/pnas.76.2.977
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Identification of inosine and hypoxanthine as endogenous ligands for the brain benzodiazepine-binding sites
Proc Natl Acad Sci U S A.
1979 Feb.
Abstract
Two endogenous ligands for the brain benzodiazepine-binding sites were isolated from bovine brain through gel filtration, paper electrophoresis, and paper chromatography. These ligands were identified as inosine and hypoxanthine, and both had a higher affinity for the brain benzodiazepine-binding sites than for benzodiazepine sites in some peripheral tissues. They did not bind to any other receptors tested, such as the opiate, muscarinic cholinergic, gamma-aminobutyric acid, and beta-adrenergic receptors. Both inosine and hypoxanthine competitively inhibited the binding of [3H]diazepam to the brain binding site.
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