Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Randomized Controlled Trial
. 2017 May 5;34(3):212-218.
doi: 10.4274/balkanmedj.2016.0782. Epub 2017 Apr 6.

Transpulmonary Hypothermia with Cooled Oxygen Inhalation Shows Promising Results as a Novel Hypothermia Technique

Yahya Ayhan Acar  1 , Banu Karakuş Yılmaz  2 , Duygu Sultan Çelik  3 , Erdem Çevik  4 , Hatice Topçu  2 , Şule Özsoy  5 , Aylin Haklıgör  6 , Orhan Çınar  7
Affiliations
Randomized Controlled Trial

Transpulmonary Hypothermia with Cooled Oxygen Inhalation Shows Promising Results as a Novel Hypothermia Technique

Yahya Ayhan Acar et al. Balkan Med J. .

Abstract

Background: Therapeutic hypothermia was showed to improve neurologic outcome but current therapeutic hypothermia techniques have limitations. Novel techniques such as transpulmonary hypothermia with cooled oxygen inhalation may be beneficial.

Aims: To evaluate the performance of transthoracic hypothermia with cooled medical oxygen inhalation as a therapeutic hypothermia method.

Study design: Animal experimentation.

Methods: A total of 36 adult male Wistar-Hannover rats were used in this research. Rats were randomised into four groups: group 1, Cooled oxygen group; group 2, IV cold fluid group; group 3, Surface cooling group; group 4, control group. No hypothermia method was applied in the control group. Hypothermia techniques were administered in the other three groups until the targeted core temperature was maintained. The target temperature was continued for one hour at 32-34 °C. After that, rats were heated up with hot blankets. Once the rectal temperature reached 38 °C, rats were euthanised. The main outcomes were the rate of temperature decrease (°C per minute) (S) and the time required to reach the target body temperature (T).

Results: All rats survived the study protocol. When compared to the control group, T and S values were better in the cooled medical oxygen inhalation group (p<0.001). The IV cold fluid group had lower S values and higher T values compared to the cooled oxygen group (p<0.001, and p=0.003, respectively). There was no meaningful pathology in the histological samples in any group.

Conclusion: As an easy-to-use and inexpensive method, cooled oxygen inhalation may be a beneficial hypothermia technique.

Keywords: Induced hypothermia; pulmonary ventilation oxygen..

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest: No conflict of interest was declared by the authors.

Figures

Figure 1
Figure 1. Schematic drawing of interventions and devices used during the study protocol.
Figure 2
Figure 2. The time-line diagram of the study.
Figure 3
Figure 3. Cooled oxygen inhalation groups showed temperature decreases at higher rates compared to the control group.
Figure 4
Figure 4. Mean rectal temperature changes of all groups in time (minutes). O2: transpulmonary hypothermia group; IV: intravenous cold fluid group; Ext: surface cooling group; Cont: control group
Figure 5
Figure 5. Histological examination of the kidney, liver, and lung tissues obtained from cooled oxygen group. Histopathological view of liver without any change (haematoxylin-eosin x100) (a). Renal tissue with normal glomeruli and tubules (haematoxylin-eosin x100) (b). Bronchial structures with slight inflammation (toluidine blue x100) (c). Minimal perivascular oedema in the lung tissue (toluidine blue x200) (d).
See this image and copyright information in PMC

References

    1. Moore EM, Nichol AD, Bernard SA, Bellomo R. Therapeutic hypothermia: benefits, mechanisms and potential clinical applications in neurological, cardiac and kidney injury. Injury. 2011;42:843–54. - PubMed
    1. Hypothermia after Cardiac Arrest Study Group. Mild therapeutic hypothermia to improve the neurologic outcome after cardiac arrest. N Engl J Med. 2002;346:549–56. - PubMed
    1. Peberdy MA, Callaway CW, Neumar RW, Geocadin RG, Zimmerman JL, Donnino M, et al. Part 9: post-cardiac arrest care: 2010 American Heart Association Guidelines for Cardiopulmonary Resuscitation and Emergency Cardiovascular Care. Circulation. 2010;122(18 Suppl 3):768–86. - PubMed
    1. Callaway CW, Donnino MW, Fink EL, Geocadin RG, Golan E, Kern KB, et al. Part 8: Post-Cardiac Arrest Care: 2015 American Heart Association Guidelines Update for Cardiopulmonary Resuscitation and Emergency Cardiovascular Care. Circulation. 2015;132(18 Suppl 2):465–82. - PMC - PubMed
    1. Donnino MW, Andersen LW, Berg KM, Reynolds JC, Nolan JP, Morley PT, et al. T Temperature Management After Cardiac Arrest: An Advisory Statement by the Advanced Life Support Task Force of the International Liaison Committee on Resuscitation and the American Heart Association Emergency Cardiovascular Care Committee and the Council on Cardiopulmonary, Critical Care, Perioperative and Resuscitation. Resuscitation. 2016;98:97–104. - PubMed

Publication types