Atypical Chemokine Receptor 1 Polymorphism can not Affect Susceptibility to Hepatitis C Virus

Abstract

Background: Hepatitis C virus has infected 130 to 150 million individuals globally. Atypical chemokine receptor 1 has become a focus of research because of its diverse roles in different diseases. However, little is known regarding the association of atypical chemokine receptor 1 polymorphism with susceptibility to hepatitis C virus.

Aims: To determine the association of an atypical chemokine receptor 1 polymorphism (rs12075) with hepatitis C virus susceptibility.

Study design: Case-control study.

Methods: We collected blood samples from 231 patients infected with hepatitis C virus and 239 blood donors as control subjects. Genotyping of atypical chemokine receptor 1 was performed using a 5'-nuclease assay with TaqMan-minor groove binding probes. Comparisons between hepatitis C virus-infected patients and control subjects were assessed using Fisher's exact test.

Results: The genotype frequencies of FY*A/FY*A, FY*A/FY*B and FY*B/FY*B were 86.1%, 13.9% and 0% in the patient group, and 86.2%, 13.4% and 0.4% in the control group, respectively. The difference in atypical chemokine receptor 1 genotype frequencies between hepatitis C virus-infected patients and control group was not significant (p=1.00, OR=1.004, 95% CI=0.594-1.695). FY*A and FY*B allele frequencies were 93.1% and 6.9% in the patient group, and 92.9% and 7.1% in the control group, respectively. The difference in atypical chemokine receptor 1 allele frequencies between hepatitis C virus-infected patients and the control group was not significant (p=1.00, OR=0.972, 95% CI=0.589-1.603).

Conclusion: Our result indicates that atypical chemokine receptor 1 polymorphism (rs12075) does not affect susceptibility to hepatitis C virus.

Keywords: Atypical chemokine receptor 1; hepatitis C virus susceptibility.; polymorphism.

Figure 1. Representative amplification curves of homozygous FY*A/FY*A (a), homozygous FY*B/FY*B (b) and heterozygous FY*A/FY*B (c). End-point fluorescent signals from several samples (d) Homozygous FY*A/FY*A showed an increased fluorescence along the Y-axis, homozygous FY*B/FY*B along the X-axis, whereas heterozygous FY*A/FY*B showed an increase in fluorescence intensity along both the X-axis and the Y-axis. Blue curves: fluorescent intensity of dye FAM. Red curves: fluorescent intensity of dye VIC. NTC: No Template Control.