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. 1988 Oct;170(10):4972-5.
doi: 10.1128/jb.170.10.4972-4975.1988.

Simple assay for quantitation of Tn5 inversion events in Escherichia coli and use of the assay in determination of plasmid copy number

P C Weber  1 , M Levine, J C Glorioso
Affiliations

Simple assay for quantitation of Tn5 inversion events in Escherichia coli and use of the assay in determination of plasmid copy number

P C Weber et al. J Bacteriol. 1988 Oct.

Abstract

In this report, we describe a simple method for measuring the frequency of sequence inversion in the transposable element Tn5 as a result of recombination across its duplicated IS50 elements. The structure of Tn5 was manipulated so that the neomycin phosphotransferase gene of the transposon would be expressed only if a sequence inversion event occurred. This highly sensitive assay also served as the basis for a novel means of estimating plasmid copy number.

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