doi: 10.1016/s0021-9673(00)94778-2.
High-performance immobilized-metal-ion affinity chromatography of peptides and proteins
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- PMID: 2844839
- DOI: 10.1016/s0021-9673(00)94778-2
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High-performance immobilized-metal-ion affinity chromatography of peptides and proteins
J Chromatogr.
.
Abstract
The present status of the undeveloped branch of high-performance immobilized-metal-ion affinity chromatography (HPIMAC) is reviewed. As demonstrated, high resolution of peptides and protein mixtures can be obtained. Under specified conditions, more than 1000 runs can be made repeatedly on a single column without much change in pattern or recovery. Occasionally, 10(3)- to 10(4)-fold purification of a protein can be obtained in a single chromatographic run.
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