A Study of Single Nucleotide Polymorphisms of Tumour Necrosis Factor α-1031 And Tumour Necrosis Factor β+ 252 in Chronic Rhinosinusitis

Abstract

Objectives: This case-controlled study aimed to identify the association of tumor necrosis factor (TNF)α-1031 and TNFβ+ 252 gene polymorphisms between chronic rhinosinusitis (CRS) and healthy controls. Another purpose of this study was to investigate the associations of these gene polymorphisms with factors related to CRS.

Methods: All deoxyribonucleic acid (DNA) samples were genotyped for TNFα-1031 and TNFβ+252 genes by mean of polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP). The statistical analysis were carried out using chi-square test or Fisher exact test to determine the associations of these gene polymorphisms in CRS. Multiple logistic regression was performed to evaluate the associations of these gene polymorphisms in CRS and its related risk factors.

Results: The genotype and allele frequencies of TNFα-1031 and TNFβ+252 gene did not show any significant associations between CRS and healthy controls. However, a significantly statistical difference of TNFα-1031 was observed in CRS participants with atopy (P-value, 0.045; odds ratio, 3.66) but not in CRS with asthma or aspirin intolerance.

Conclusion: Although the presence of TNFα-1031 and TNFβ+252 gene polymorphisms did not render any significant associations between CRS and healthy control, this study suggests that TNFα-1031 gene polymorphisms in CRS patients with atopy may be associated with increase susceptibility towards CRS.

Keywords: Rhinosinusitis; Single Nucleotide Polymorphism; Tumour Necrosis Factors.

Fig. 1.

A restriction fragment length polymorphism analysis of tumor necrosis factor-alpha-1031 gene. Lane 2, 4, 5, and 7: homozygous wild-type (TT); lane 1, 3, and 6: heterozygous mutant-type (TC); M: 100 bp DNA ladder.

Fig. 2.

A restriction fragment length polymorphism analysis of tumor necrosis factor-beta+252 gene. Lane 3, 4, and 7: homozygous wildtype (AA); lane 1, 5, and 6: heterozygous mutant-type (AG); lane 2: homozygous mutant-type (GG); M: 100 bp DNA ladder.