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. 1988 Nov;62(11):4224-31.
doi: 10.1128/JVI.62.11.4224-4231.1988.

Structural analysis of a hepatitis B virus genome integrated into chromosome 17p of a human hepatocellular carcinoma

Y Z Zhou  1 , B L Slagle, L A Donehower, P vanTuinen, D H Ledbetter, J S Butel
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Structural analysis of a hepatitis B virus genome integrated into chromosome 17p of a human hepatocellular carcinoma

Y Z Zhou et al. J Virol. 1988 Nov.

Abstract

Hepatitis B virus (HBV) is clearly a factor in the development of hepatocellular carcinoma, but its mechanism of action remains obscure. One possibility is that the HBV integration event alters the expression of a nearby growth-regulatory cellular gene. A 9-kilobase (kb) DNA fragment containing an HBV insert plus flanking cellular sequences was cloned from a hepatoma specimen from Shanghai, People's Republic of China. Restriction mapping of the insert revealed a large inverted repeat structure consisting of both viral sequences (encompassing all of the core and pre-S regions and portions of the X and S genes) and at least 3 kb of unique cellular sequences. The virus-cell junction mapped 11 nucleotides from the DR1 region, in a position within the HBV X gene and included in the cohesive overlap region. A probe generated from 1.0 kb of the flanking cellular DNA mapped the viral insert to chromosome 17 in the region designated 17p11.2-17p12, which is near the human proto-oncogene p53. Sequence data from a portion of the flanking cellular DNA revealed a stretch of approximately 70 base pairs that showed highly significant homology with a conserved region of a number of functional mammalian DNAs, including the human autonomously replicating sequence 1 (ARS1).

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