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. 1988 Nov;62(11):4387-92.
doi: 10.1128/JVI.62.11.4387-4392.1988.

Vesicular stomatitis virus M protein in the nuclei of infected cells

D S Lyles  1 , L Puddington, B J McCreedy Jr
Affiliations

Vesicular stomatitis virus M protein in the nuclei of infected cells

D S Lyles et al. J Virol. 1988 Nov.

Abstract

The M protein of vesicular stomatitis virus (VSV) was localized in the nuclei and cytoplasm of VSV-infected cells by subcellular fractionation and immunofluorescence microscopy. Nuclei isolated from VSV-infected Friend erythroleukemia cells were fractionated into a nuclear membrane and a nucleoplasm fraction by DNase digestion and differential centrifugation. G protein was present in the membrane fraction, and M protein was present in the nucleoplasm fraction. Immunofluorescence detection of M protein in the nucleus required that fixed cells be permeabilized with higher concentrations of detergent than were required for detection of M protein in the cytoplasm of VSV-infected BHK cells.

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References

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