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. 2017 Apr:52:17-20.
doi: 10.1016/j.placenta.2017.02.010. Epub 2017 Feb 13.

Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE

Jill L Reiter  1 , Holli M Drendel  2 , Sujata Chakraborty  2 , Megan M Schellinger  3 , Men-Jean Lee  3 , Gil Mor  4
Affiliations
Free article

Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE

Jill L Reiter et al. Placenta. 2017 Apr.
Free article

Abstract

Immortalization of primary cells with telomerase is thought to maintain normal phenotypic properties and avoid chromosomal abnormalities and other cancer-associated changes that occur following simian virus 40 tumor antigen (SV40 Tag) induced immortalization. However, we report that the human telomerase reverse transcriptase (hTERT)-immortalized SWAN-71 trophoblast cell line has a near pentaploid 103∼119,XXXX[cp20] karyotype. Additionally, DNA typing analysis indicated that SWAN-71 cells have acquired microsatellite instability. In comparison, the post-crisis SV40-transformed trophoblast cell line 3A-subE was hypertriploid 69∼81,XX[cp20]. Both cell lines contained multiple specific clonal rearrangements. These findings emphasize the need to monitor for genetic instability in hTERT-immortalized cells.

Keywords: Cellular immortalization; Karyotype; Polyploidy; Short tandem repeat profile; Telomerase; Trophoblast cell lines.

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