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. 2017 Apr 29:23:2059-2064.
doi: 10.12659/msm.901381.

Boschniakia Rossica Polysaccharide Triggers Laryngeal Carcinoma Cell Apoptosis by Regulating Expression of Bcl-2, Caspase-3, and P53

Chunping Yao  1   2 , Xiujuan Cao  2 , Zheng Fu  3 , Jing Tian  4 , Wei Dong  2 , Jin Xu  2 , Kang An  5 , Limin Zhai  2 , Jinming Yu  2
Affiliations

Boschniakia Rossica Polysaccharide Triggers Laryngeal Carcinoma Cell Apoptosis by Regulating Expression of Bcl-2, Caspase-3, and P53

Chunping Yao et al. Med Sci Monit. .

Retraction in

Abstract

BACKGROUND Laryngeal cancer is a malignant head and neck tumor with high morbidity and high mortality in humans. Recently, treatments with Chinese medicines and their extracts have gradually received great attention, and studies suggest that Boschniakia rossica polysaccharide (BRP) has potential anti-tumor activity. Therefore, this study investigating the role of BRP in inducing apoptosis in human laryngeal carcinoma cells. MATERIAL AND METHODS The BRP was extracted with organic solvent and HR column. We treated Hep2 laryngeal carcinoma cells with different concentrations of BRP, then assessed cell growth inhibition rate by flow cytometry and apoptosis index by TUNEL staining. The protein expression of p53, Bcl-2, Bax, and caspase-3 were analyzed by Western blot. RESULTS Flow cytometry results showed that BRP inhibited Hep2 cell proliferation in a dose-dependent manner (p<0.05), and TUNEL staining indicated that BRP significantly increased Hep2 apoptosis index (p<0.05). Western blot results showed that the expression levels of p53 and activation of caspase-3 in Hep2 cells were significantly up-regulated (p<0.05), while the expression of Bcl-2 was significantly down-regulated (p<0.05). CONCLUSIONS BRP might induce cell apoptosis by regulating the expression level of cell apoptosis-associated proteins, suggesting strong anti-laryngeal cancer activity.

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Conflict of interest statement

Disclosure of conflict of interest

None.

Figures

Figure 1
Figure 1
The growth inhibition rate changes at 48 h after adding various concentrations of BRP.
Figure 2
Figure 2
The apoptosis index of Hep 2 cells after treatment with BRP (200 mg/L). (A) TUNEL staining on day 4; arrows indicate nuclei stained brown, representing the apoptotic cells. (B) The apoptosis index of Hep 2 cells; each group underwent 6 parallel experiments. TUNEL staining was performed on days 1, 2, 3, and 4. Under an optical microscope, cells were photographed randomly, and 5 high-power fields were selected to count the apoptotic cells and total cell number; the apoptosis index is the ratio between these 2 values. * Compared with the control group, p<0.05.
Figure 3
Figure 3
Expression of P53, Bcl-2, and Caspase-3 in different concentrations of BRP-treated Hep2 cells. (A) Immunoblot results of P53, Bcl-2, and Caspase-3 proteins; (B) The statistical results of expression of Bcl-2 protein; (C) The statistical results of expression level of p53 protein; (D) The statistical results of expression of Caspase-3 protein. Independent experiments were repeated 3 times. * Compared with the control group, p<0.05.
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