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. 2017 Apr 19:11:1267-1272.
doi: 10.2147/DDDT.S131582. eCollection 2017.

Anti-platelet effects of anti-glaucomatous eye drops: an in vitro study on human platelets

Marilita M Moschos  1   2 , Giannis A Moustafa  1 , Vasiliki D Papakonstantinou  3 , Michael Tsatsos  4 , Konstantinos Laios  1 , Smaragdi Antonopoulou  3
Affiliations

Anti-platelet effects of anti-glaucomatous eye drops: an in vitro study on human platelets

Marilita M Moschos et al. Drug Des Devel Ther. .

Abstract

Purpose: Altered platelet aggregability has been implicated in the pathogenesis of glaucoma. This study aims to investigate the anti-platelet potential of intraocular pressure lowering drops, with the possibility of establishing it as an additional mechanism of anti-glaucomatous action.

Materials and methods: The anti-aggregating effects of a series of anti-glaucomatous eye drops were determined on human platelets in the platelet aggregation model, using four known aggregating factors (platelet activating factor [PAF], adenosine diphosphate [ADP], thrombin receptor-activating peptide [TRAP], and arachidonic acid [AA]).

Results: Almost all of the tested samples inhibited platelet aggregation induced by PAF, ADP, TRAP, and AA, except for Alphagan, which did not demonstrate inhibition of ADP- and TRAP-induced aggregation at a wide range of concentrations. Trusopt, Betoptic, and Azarga eye drops were the most potent inhibitors of all four aggregating factors, while Alphagan was the least potent (P<0.05).

Conclusion: This study shows that anti-glaucomatous eye drops possess anti-platelet effects, and this was shown for the first time by experimenting on human platelets.

Keywords: PAF; TRAP; aggregation; eye; glaucoma; platelet.

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Conflict of interest statement

Disclosure The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Graphical depiction of the IC50 values for each sample after stimulation with each of the four aggregating factors. Abbreviations: PAF, platelet activating factor; ADP, adenosine diphosphate; TRAP, thrombin receptor-activating peptide; AA, arachidonic acid.
See this image and copyright information in PMC

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