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. 2017 Jun 27;61(7):e00454-17.
doi: 10.1128/AAC.00454-17. Print 2017 Jul.

NDM-5 and OXA-181 Beta-Lactamases, a Significant Threat Continues To Spread in the Americas

Laura J Rojas  1   2 , Andrea M Hujer  1   3 , Susan D Rudin  1   3 , Meredith S Wright  4 , T Nicholas Domitrovic  1   3 , Steven H Marshall  1 , Kristine M Hujer  1   3 , Sandra S Richter  5 , Eric Cober  6 , Federico Perez  7   3 , Mark D Adams  4 , David van Duin  8 , Robert A Bonomo  9   1   3   2   10   11   12
Affiliations

NDM-5 and OXA-181 Beta-Lactamases, a Significant Threat Continues To Spread in the Americas

Laura J Rojas et al. Antimicrob Agents Chemother. .

Abstract

Among Gram-negative bacteria, carbapenem-resistant infections pose a serious and life-threatening challenge. Here, the CRACKLE network reports a sentinel detection and characterization of a carbapenem-resistant Klebsiella pneumoniae ST147 isolate harboring blaNDM-5 and blaOXA-181 from a young man who underwent abdominal surgery in India. blaNDM-5 was located on an IncFII plasmid of ≈90 kb, whereas blaOXA-181 was chromosomally encoded. Resistome and genome analysis demonstrated multiple copies of the transposable element IS26 and a "hot-spot region" in the IncFII plasmid.

Keywords: Klebsiella pneumoniae; NDM-5; OXA-181.

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Figures

FIG 1
FIG 1
(A) Coverage of Illumina reads from K. pneumoniae isolates CR Kp-1 and CR Kp-2 mapped using Bowtie2 (33) across the reference IncFII plasmid pCC1409 (KT725789.1). (B) Organization of the 25-kb blaNDM-5-containing region of the IncFII plasmid present only in CR Kp-1. This entire region was excised out of the IncFII plasmid present in CR Kp-2.
See this image and copyright information in PMC

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