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. 1988 Oct;7(10):2961-6.
doi: 10.1002/j.1460-2075.1988.tb03158.x.

Immunoelectron microscopic localization of ubiquitin in hepatoma cells

A L Schwartz  1 , A Ciechanover, R A Brandt, H J Geuze
Affiliations

Immunoelectron microscopic localization of ubiquitin in hepatoma cells

A L Schwartz et al. EMBO J. 1988 Oct.

Abstract

Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.

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