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. 1988 Sep;26(9):1874-7.
doi: 10.1128/jcm.26.9.1874-1877.1988.

Improved method for azole antifungal susceptibility testing

M A Gordon  1 , E W Lapa, P G Passero
Affiliations

Improved method for azole antifungal susceptibility testing

M A Gordon et al. J Clin Microbiol. 1988 Sep.

Abstract

A reproducible method is described for the determination of the MICs of ketoconazole, miconazole, fluconazole, and itraconazole with sharp endpoints when employed with either yeasts or molds. A semisolid medium is used with controlled pH and standardized inoculum. The time of reading results is a critical factor in the conduct of this test. The medium is simple to prepare and has a relatively long refrigerator shelf life in a user-ready state, requiring only the addition of a freshly prepared inoculum after restoration to room temperature.

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