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. 2017 Apr 30;22(5):721.
doi: 10.3390/molecules22050721.

One-Step Synthesis of Silver Nanoparticles on Polydopamine-Coated Sericin/Polyvinyl Alcohol Composite Films for Potential Antimicrobial Applications

Rui Cai  1 , Gang Tao  2 , Huawei He  3 , Kai Song  4 , Hua Zuo  5 , Wenchao Jiang  6 , Yejing Wang  7
Affiliations

One-Step Synthesis of Silver Nanoparticles on Polydopamine-Coated Sericin/Polyvinyl Alcohol Composite Films for Potential Antimicrobial Applications

Rui Cai et al. Molecules. .

Abstract

Silk sericin has great potential as a biomaterial for biomedical applications due to its good hydrophilicity, reactivity, and biodegradability. To develop multifunctional sericin materials for potential antibacterial application, a one-step synthesis method for preparing silver nanoparticles (AgNPs) modified on polydopamine-coated sericin/polyvinyl alcohol (PVA) composite films was developed. Polydopamine (PDA) acted as both metal ion chelating and reducing agent to synthesize AgNPs in situ on the sericin/PVA composite film. Scanning electron microscopy and energy dispersive spectroscopy analysis revealed that polydopamine could effectively facilitate the high-density growth of AgNPs as a 3-D matrix. X-ray diffractometry studies suggested the synthesized AgNPs formed good face-centered cubic crystalline structures. Contact angle measurement and mechanical test indicated AgNPs modified PDA-sericin/PVA composite film had good hydrophilicity and mechanical property. The bacterial growth curve and inhibition zone assays showed the AgNPs modified PDA-sericin/PVA composite film had long-term antibacterial activities. This work develops a new method for the preparation of AgNPs modified PDA-sericin/PVA film with good hydrophilicity, mechanical performance and antibacterial activities for the potential antimicrobial application in biomedicine.

Keywords: antibacterial activity; one-step synthesis; polydopamine; polyvinyl alcohol; sericin; silver nanoparticles.

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Conflict of interest statement

The authors declare that they have no competing interests, including specific financial interests and relationships and affiliations relevant to the subject of this manuscript.

Figures

Figure 1
Figure 1
The flow diagram illustrates the preparation and antimicrobial analysis of AgNPs-PDA-SS/PVA composite film.
Figure 2
Figure 2
SEM images of SS/PVA film (a) and PDA-coated SS/PVA films prepared with dopamine concentration of 0.5 (b), 1.0 (c), 2.0 (d), 3.0 (e) and 5.0 mg/mL (f), respectively.
Figure 3
Figure 3
Field emission scanning electron microscope images of AgNPs-modified PDA-SS/PVA film (a) and (b). Inset in (a), particle size distribution of AgNPs. The red arrows in (b) indicated the different morphologies of the synthesized AgNPs. (c) EDS spectrum of a selected area of AgNPs-PDA-SS/PVA film. (d) XRD patterns of pure sericin (d1), SS/PVA film (d2), PDA-SS/PVA film (d3) and AgNPs modified PDA-SS/PVA film (d4).
Figure 4
Figure 4
FT-IR spectra of pure sericin (a), SS/PVA composite film (b), PDA-SS/PVA composite film (c) and AgNPs-modified PDA-SS/PVA composite film (d).
Figure 5
Figure 5
Water contact angles of SS/PVA (a), PDA-SS/PVA (b), and AgNPs-PDA-SS/PVA (c). Swelling ratio of these films (d) (n = 3 per group; * indicates significant differences compared with SS/PVA film at p < 0.05, ** indicates significant differences compared with SS/PVA film at p < 0.01).
Figure 6
Figure 6
Mechanical properties of the films: (a) tensile strength and (b) elongation at break (n = 3 per group; * indicates p < 0.05).
Figure 7
Figure 7
Inhibition zone assays of SS/PVA, PDA-SS/PVA, AgNPs-PDA-SS/PVA against E. coli (a) and S. aureus (b).
Figure 8
Figure 8
Growth curves of E. coli (a) and S. aureus (b). Bacteria without treatment (a1, b1); Bacteria treated with SS/PVA films (a2, b2), PDA-SS/PVA films (a3, b3) and AgNPs-PDA-SS/PVA films (a4, b4). Long-term bactericidal activity against E. coli (c) and S. aureus (d) (n = 3 per group; ** indicates p < 0.01).
Figure 9
Figure 9
Mass loss kinetics of AgNPs-PDA-SS/PVA films at different pH values.
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