Fine mapping of the immediate-early gene of the Indiana-Funkhauser strain of pseudorabies virus
- PMID: 2846885
- PMCID: PMC253594
- DOI: 10.1128/JVI.62.12.4763-4766.1988
Fine mapping of the immediate-early gene of the Indiana-Funkhauser strain of pseudorabies virus
Abstract
The immediate-early gene transcript of pseudorabies virus was found to be 5.6 kilobases and colinear with the DNA genome. Transcription initiation started in the BamHI E fragment, 45 nucleotides from the BamHI-E/BamHI-I junction. The first AUG codon was located in BamHI-I, 260 nucleotides from the mRNA initiation site, and a potential TGA termination codon was located in BamHI-J, 161 nucleotides from the BamHI-I/BamHI-J junction. The AATAAA polyadenylation signal was 30 bases from the stop codon, while the actual polyadenylation site was 18 bases further downstream.
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