Cytochrome P-450 1B1 Leu432Val Polymorphism Does Not Show Association With Breast Cancer in Northern Iranian Women With a History of Infertility

Abstract

The Cytochrome P-4501B1 (CYP1B1) Leu432Val polymorphism has been previously shown to be associated with some types of cancer and affects CYP1B1-mediated metabolism of various infertility drugs. To establish the frequency of CYP1B1 Leu432Val polymorphism among women with a history of infertility drug use, we studied the genotypes of 147 patients with breast cancer with a history of infertility and 150 cancer-free, infertile women (control group) in Northern Iran. A polymerase chain reaction-based restriction fragment length polymorphism assay was used to detect GG (Val/Val), CG (Leu/Val), and CC (Leu/Leu) genotype frequencies, which did not vary significantly between the 2 patient groups (P = .847). We established for the first time that the incidence of CYP1B1 Leu432Val polymorphism is 46.6% among women with infertility history and breast cancer in Northern Iran. Finally, our results do not show any significant association between CYP1B1 Leu432Val polymorphism and breast cancer in infertile women in this region, who have also received infertility treatment.

Keywords: Cytochrome P-4501B1; Iran; breast cancer; drugs; infertility; polymorphism.

Figure 1.

Polymerase chain reaction-restriction fragment length polymorphism to identity CYP1B1 polymorphic genotypes via restriction digest with EcoR57I. Lane 1: mutant homozygous genotype Val/Val (271 bp), lane 2: 50-bp reference ladder, lanes 3 and 4: wild-type homozygous Leu/Leu genotype (166 and 105 bp), and lane 5: heterozygous genotype Leu/Val (271, 166, and 105 bp).

Figure 2.

CYP1B1 Lue432Val genotype frequencies in infertile women with (case) and without (control) breast cancer.