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. 2017 Jul;27(4):1225-1231.
doi: 10.1007/s10895-017-2109-z. Epub 2017 May 3.

Novel Application of Red Fluorescent Protein (DsRed-Express) for the Study of Functional Dynamics of Nuclear Receptors

Amit K Dash  1 , Ashutosh S Yende  1 , Rakesh K Tyagi  2
Affiliations

Novel Application of Red Fluorescent Protein (DsRed-Express) for the Study of Functional Dynamics of Nuclear Receptors

Amit K Dash et al. J Fluoresc. 2017 Jul.

Abstract

Arrivals of fluorescent proteins have revolutionized the way we do research in the areas of molecular cell biology. In the present study, we have successfully exploited the multimer-forming property of Red Fluorescent Protein (RFP) in living cells to preferentially shift the unliganded nuclear receptors from the nuclear to the cytoplasmic compartment. Subsequently, these cytoplasmic-shifted unliganded receptors could be induced to translocate into the nucleus by their ligands. Though the multimerization of RFP as a protein-tag is viewed as a disadvantage, we have exploited and projected this property towards novel applications in validating the clinical drugs, herbal compounds, metabolic disruptors etc. Such cytoplasmic shifted transcription factors can offer a unique opportunity to study receptor-ligand interactions and functional dynamics by analyzing ligand-mediated receptor translocation from cytoplasmic compartment to the nucleus of living cells.

Keywords: CAR; GFP; PXR; RFP; RXRα; Subcellular localization.

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References

    1. Nat Methods. 2009 Oct;6(10):713-17 - PubMed
    1. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60 - PubMed
    1. Curr Protoc Cell Biol. 2005 Jul;Chapter 21:Unit 21.4 - PubMed
    1. Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11990-5 - PubMed
    1. Science. 1994 Feb 11;263(5148):802-5 - PubMed

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