Review
doi: 10.1007/978-1-4939-6887-9_1.
Recombinant Protein Expression in E. coli : A Historical Perspective
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- PMID: 28470595
- DOI: 10.1007/978-1-4939-6887-9_1
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Review
Recombinant Protein Expression in E. coli : A Historical Perspective
Methods Mol Biol.
2017.
Abstract
This introductory chapter provides a brief historical survey of the key elements incorporated into commonly used E. coli-based expression systems. The highest impact in expression technology is associated with innovations that were based on extensively studied biological systems, and where the tools were widely distributed in the academic community.
Keywords: E. coli; Expression vectors; Promoter; Protein engineering; Recombinant protein.
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