doi: 10.1007/978-1-4939-6887-9_17.
Expression of Prokaryotic Integral Membrane Proteins in E. coli
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- PMID: 28470611
- DOI: 10.1007/978-1-4939-6887-9_17
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Expression of Prokaryotic Integral Membrane Proteins in E. coli
Methods Mol Biol.
2017.
Abstract
Production of prokaryotic membrane proteins for structural and functional studies in E. coli can be parallelized and miniaturized. All stages from cloning, expression, purification to detergent selection can be investigated using high-throughput techniques to rapidly and economically find tractable targets.
Keywords: Detergent assay; High throughput; Membrane proteins; Multiwell.
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