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. 2017:1586:265-278.
doi: 10.1007/978-1-4939-6887-9_17.

Expression of Prokaryotic Integral Membrane Proteins in E. coli

James D Love  1   2
Affiliations

Expression of Prokaryotic Integral Membrane Proteins in E. coli

James D Love. Methods Mol Biol. 2017.

Abstract

Production of prokaryotic membrane proteins for structural and functional studies in E. coli can be parallelized and miniaturized. All stages from cloning, expression, purification to detergent selection can be investigated using high-throughput techniques to rapidly and economically find tractable targets.

Keywords: Detergent assay; High throughput; Membrane proteins; Multiwell.

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