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Review
. 2017 Sep;84(9):814-821.
doi: 10.1002/mrd.22829. Epub 2017 Jun 8.

Developmental competence of porcine genome-edited zygotes

Maria A Gil  1 , Cristina A Martinez  1 , Alicia Nohalez  1 , Inmaculada Parrilla  1 , Jordi Roca  1 , Jun Wu  2 , Pablo J Ross  3 , Cristina Cuello  1 , Juan C Izpisua  2 , Emilio A Martinez  1
Affiliations
Review

Developmental competence of porcine genome-edited zygotes

Maria A Gil et al. Mol Reprod Dev. 2017 Sep.

Abstract

Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site-specific nucleases-including ZFNs, TALENs, and the CRISPR/Cas9 system-has popularized targeted zygote genome editing via one-step microinjection in several mammalian species. Here, we review methods to optimize the developmental competence of genome-edited porcine embryos and strategies to improve the zygote genome-editing efficiency in pigs.

Keywords: CRISPR/Cas9; TALEN; ZFN; cytoplasmic microinjection; in vitro-produced zygotes; in vivo-derived zygotes.

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