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. 1988 Nov;85(22):8506-10.
doi: 10.1073/pnas.85.22.8506.

In vitro-derived leukemic erythroid cell lines induced by a raf- and myc-containing retrovirus differentiate in response to erythropoietin

S P Klinken  1 , N A Nicola, G R Johnson
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In vitro-derived leukemic erythroid cell lines induced by a raf- and myc-containing retrovirus differentiate in response to erythropoietin

S P Klinken et al. Proc Natl Acad Sci U S A. 1988 Nov.

Abstract

In vitro infection of murine fetal liver cells with a retrovirus containing v-raf and v-myc oncogenes has produced continuous lines of immature erythroid cells that are leukemogenic. These cells synthesized a factor that stimulated their growth in vitro before autonomous variants emerged. Approximately 1000 high-affinity erythropoietin receptors could be detected per cell, and the hormone induced terminal differentiation in these cells. The lines were generated at an extremely low frequency (approximately 1 in 10(7) cells), suggesting that the combination of raf and myc is insufficient to develop erythroid cell lines and that additional events are necessary for transformation.

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References

    1. Mol Cell Biol. 1982 Jun;2(6):617-24 - PubMed
    1. Nature. 1986 Aug 21-27;322(6081):748-50 - PubMed
    1. J Immunol. 1986 Jul 15;137(2):679-88 - PubMed
    1. J Cell Physiol. 1986 Aug;128(2):180-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6480-4 - PubMed

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