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. 2017 Apr 1;1(4):0092.
doi: 10.1038/s41559-017-0092.

Organelles that illuminate the origins of Trichomonas hydrogenosomes and Giardia mitosomes

Michelle M Leger  1 , Martin Kolisko  1 , Ryoma Kamikawa  2 , Courtney W Stairs  1 , Keitaro Kume  3 , Ivan Čepička  4 , Jeffrey D Silberman  5 , Jan O Andersson  6 , Feifei Xu  6 , Akinori Yabuki  7 , Laura Eme  1 , Qianqian Zhang  8 , Kiyotaka Takishita  7 , Yuji Inagaki  3   9 , Alastair G B Simpson  10 , Tetsuo Hashimoto  3   9 , Andrew J Roger  1
Affiliations

Organelles that illuminate the origins of Trichomonas hydrogenosomes and Giardia mitosomes

Michelle M Leger et al. Nat Ecol Evol. .
No abstract available

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Conflict of interest statement

Competing Financial Interests Statement The authors declare that they have no competing financial interests.

Figures

Fig. 1
Fig. 1
Phylogeny of Metamonada and distribution of MRO-localising proteins. a. PhyloBayes tree of eukaryotes inferred from the phylogenomic dataset of 159 proteins (39,089 sites) and 94 taxa. The taxa other than Metamonada are not shown in detail. The topology shown was recovered by 3/4 PhyloBayes chains, and in all ML analyses. Parasitic species and lineages are highlighted in red. The bar graph indicates the percent coverage of the amino acid sites used in the phylogenomic analysis present in each transcriptome. See Supplementary Figs. 1, 2 and 3 for more detail and support values. b. Distribution of MRO-targeted proteins in Metamonada. L, H, P, and T: L, H, P, and T proteins of the Glycine cleavage system, SHMT: Serine hydroxymethyltransferase, NuoE: NADH:ubiquinone oxidoreductase 24 kD subunit, NuoF: NADH:ubiquinone oxidoreductase, NADH-binding 51 kD subunit, Fdx: ferredoxin, HydA: [FeFe]-hydrogenase, HydE-G: [FeFe]-hydrogenase maturation proteins E-G, SCSa: Succinyl-CoA synthase alpha subunit, SCSb: Succinyl-CoA synthase beta subunit, ASCT: Acetate:succinyl-CoA transferase, ACS1 and ACS2: acetyl-CoA synthase 1 and acetyl-CoA synthase 2. Note that Monocercomonoides sp. has undergone a complete secondary loss of MROs.
Fig. 2
Fig. 2
Predicted mitochondrial protein import proteins in metamonads. Blue +: present; white −: absent from genome; grey, no symbols: absent from transcriptome. Data from Saccharomyces cerevisiae and Arabidopsis thaliana are provided for comparison.
Fig. 3
Fig. 3
Evolutionary transitions of metabolic pathways of MROs from the common ancestor of Parabasalia and Fornicata to representative extant species. a. Ancestor of Parabasalia and Fornicata. b. Ancestor of Parabasalia. c. Ancestor of Fornicata. d. Dysnectes brevis. e. Ancestor of diplomonads. f. Giardia intestinalis. g. Spironucleus salmonicida. In this figure, only glycine cleavage (via the glycine cleavage system), hydrogen production, and ATP production are shown (we cannot confidently assign cytosolic or MRO localization of proteins involved in pyruvate metabolism, other than in Giardia, Trichomonas and Spironucleus). cACS1/cACS2, cytosolic acetyl-CoA synthase 1/cytosolic acetyl-CoA synthase 2; hACS2, hydrogenosomal acetyl-CoA synthase 2.
See this image and copyright information in PMC

References

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