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. 1988 Aug 15;173(1):185-9.
doi: 10.1016/0003-2697(88)90176-5.

Determination of glucose-6-phosphatase activity using the glucose dehydrogenase-coupled reaction

M Alegre  1 , C J Ciudad, C Fillat, J J Guinovart
Affiliations

Determination of glucose-6-phosphatase activity using the glucose dehydrogenase-coupled reaction

M Alegre et al. Anal Biochem. .

Abstract

We present a method to determine glucose 6-phosphate activity. This assay measures the rate of glucose released in the glucose-6-phosphatase reaction. The glucose is oxidized to beta-D-gluconolactone by glucose dehydrogenase in a coupled reaction that uses NAD(P)+. The determination is rapid, reproducible, and does not require withdrawal, precipitation, centrifugation, or neutralization steps. This method provides a simple resolution to the problem of the nonspecific appearance of Pi, which is especially important in studies of regulation of glucose-6-phosphatase performed in the presence of ATP.

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