doi: 10.1007/978-1-4939-6877-0_7.
Determining the Frequencies of Th9 Cells from Whole Blood
Affiliations
- PMID: 28477189
- DOI: 10.1007/978-1-4939-6877-0_7
Item in Clipboard
Determining the Frequencies of Th9 Cells from Whole Blood
Methods Mol Biol.
2017.
Abstract
Th9 cells are a subset of CD4+ T cells producing the cytokine, IL-9. Th9 cells are increasingly recognized as being important player in allergy, autoimmunity, and antitumor responses. The polarization and expansion of Th9 cells requires the cytokines IL-4, TGF-β. In this chapter, we described the protocol for measuring Th9 frequencies using whole blood.
Keywords: Flow cytometry; IL-9 cytokine; Intracellular cytokine assay; Th9; Whole blood immunophenotyping.
Similar articles
-
Flow Cytometric Assessment of STAT Molecules in Th9 Cells.Methods Mol Biol. 2017;1585:127-140. doi: 10.1007/978-1-4939-6877-0_10. Methods Mol Biol. 2017. PMID: 28477192
-
T Cell Receptor and Co-Stimulatory Signals for Th9 Generation.Methods Mol Biol. 2017;1585:59-71. doi: 10.1007/978-1-4939-6877-0_5. Methods Mol Biol. 2017. PMID: 28477187
-
Polarizing Cytokines for Human Th9 Cell Differentiation.Methods Mol Biol. 2017;1585:73-82. doi: 10.1007/978-1-4939-6877-0_6. Methods Mol Biol. 2017. PMID: 28477188
-
Th9 and other IL-9-producing cells in allergic asthma.Semin Immunopathol. 2017 Jan;39(1):55-68. doi: 10.1007/s00281-016-0601-1. Epub 2016 Nov 17. Semin Immunopathol. 2017. PMID: 27858144 Review.
-
Th9 cells in the pathogenesis of EAE and multiple sclerosis.Semin Immunopathol. 2017 Jan;39(1):79-87. doi: 10.1007/s00281-016-0604-y. Epub 2016 Nov 14. Semin Immunopathol. 2017. PMID: 27844107 Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
