Perspectives for elucidating the ethylenediurea (EDU) mode of action for protection against O3 phytotoxicity

Abstract

Ethylenediurea (EDU) has been widely studied for its effectiveness to protect plants against injuries caused by surface ozone (O₃), however its mode of action remains unclear. So far, there is not a unified methodological approach and thus the methodology is quite arbitrary, thereby making it more difficult to generalize findings and understand the EDU mode of action. This review examines the question of whether potential N addition to plants by EDU is a fundamental underlying mechanism in protecting against O₃ phytotoxicity. Yet, this review proposes an evidence-based hypothesis that EDU may protect plants against O₃ deleterious effects upon generation of EDU-induced hormesis, i.e. by activating plant defense at low doses. This hypothesis challenges the future research directions. Revealing a hormesis-based EDU mode of action in protecting plants against O₃ toxicity would have further implications to ecotoxicology and environmental safety. Furthermore, this review discusses the need for further studies on plant metabolism under EDU treatment through relevant experimental approach, and attempts to set the bases for approaching a unified methodology that will contribute in revealing the EDU mode of action. In this framework, focus is given to the main EDU application methods.

Keywords: Antiozonant; Biphasic; Ethylenediurea; Hormesis; Ozone; Phytoprotectant.