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. 2017:1600:143-150.
doi: 10.1007/978-1-4939-6958-6_14.

A Bioassay for the Determination of Lipopolysaccharides and Lipoproteins

Marcus Peters  1 , Petra Bonowitz  2 , Albrecht Bufe  2
Affiliations

A Bioassay for the Determination of Lipopolysaccharides and Lipoproteins

Marcus Peters et al. Methods Mol Biol. 2017.

Abstract

The availability of convenient assays for the detection and quantification of pathogen-associated molecular patterns (PAMPs) is limited. In the case of lipopolysaccharide (LPS) the so-called LAL (limulus amebocyte lysate) test is available, an assay that is performed with the lysate of the blood of the horse shoe crab. Although a sensitive and convenient assay, it lacks specificity, since it is affected by other endotoxins like, for instance, fungal cell walls as well. Here, we describe a bioassay that can be used to detect and quantitate PAMPs in environmental samples. More specific we demonstrate the usage of TLR2 and TLR4/CD14/MD2 transfected Hek293 cells to quantitatively determine bacterial lipoproteins and LPS, respectively. We show the usefulness of these assays to measure LPS in tobacco before and after combustion.

Keywords: Bioassay; Lipopolysaccharide; Lipoprotein; Pathogen-associated molecular patterns; Toll-like receptor.

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