Long range physical map of the Wilms' tumor-aniridia region on human chromosome 11

Abstract

The relationship between genetic alterations at chromosomal band 11p13 and the WAGR (Wilms' tumor, aniridia, genitourinary anomalies, and mental retardation) syndrome is not clearly understood. To aid our understanding of this relationship, we have constructed a physical map of this region of the genome using pulsed field gel electrophoresis. Fifteen newly identified 11p13-specific probes and four previously reported probes were used to subdivide 11p13 into five intervals defined by overlapping constitutional deletions from several WAGR patients. This new repertoire of DNA probes was used to construct a physical map of this region using the infrequently cutting restriction enzymes MIuI and NotI. This map spans approximately 13 Mb and encompasses deletion and translocation breakpoints associated with genitourinary abnormalities, aniridia, and Wilms' tumor. The map also makes it possible to localize the genes for Wilms' tumor (WT) and aniridia (AN2) to a small number of specific NotI restriction fragments.