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. 2017 Mar 4;4(2):ofx046.
doi: 10.1093/ofid/ofx046. eCollection 2017 Spring.

Central Nervous System Infection Diagnosis by Next-Generation Sequencing: A Glimpse Into the Future?

Nguyen Thi Hoang Mai  1 , Nguyen Hoan Phu  2 , Le Nguyen Truc Nhu  1 , Nguyen Thi Thu Hong  1 , Nguyen Ho Hong Hanh  3 , Lam Anh Nguyet  1 , Tran My Phuong  2 , Angela McBride  1 , Do Quang Ha  1 , Ho Dang Trung Nghia  4 , Nguyen Van Vinh Chau  2 , Guy Thwaites  1   5 , Le Van Tan  1
Affiliations

Central Nervous System Infection Diagnosis by Next-Generation Sequencing: A Glimpse Into the Future?

Nguyen Thi Hoang Mai et al. Open Forum Infect Dis. .

Abstract

Japanese encephalitis virus was detected by deep sequencing for the first time in urine of a 16-year-old boy with encephalitis. Seroconversion and polymerase chain reaction analysis confirmed the metagenomics finding. Urine is useful for diagnosis of flaviviral encephalitis, whereas deep sequencing can be a panpathogen assay for the diagnosis of life-threatening infectious diseases.

Keywords: Japanese encephalitis virus; Vietnam.; deep sequencing.

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Figures

Figure 1.
Figure 1.
(A) Maximum likelihood tree based on the 1.7-kb sequence obtained from the patient’s urine sample (KY099618) and the corresponding ribonucleic acid (RNA)-dependent RNA polymerase coding region genomic region of representatives of Japanese encephalitis virus (JEV): West Nile virus (WNV) was used as an outgroup. Sequence accession numbers are in brackets. (B) Brain magnetic resonance imaging showing a lesion of the putamen with a high signal on a fluid-attenuated inversion recovery image. Abbreviations: GI-A, genotype I-A; GI-B, genotype I-B; GII, genotype II; GIII, genotype III; GIV, genotype IV; GV, genotype V.
See this image and copyright information in PMC

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