Physicochemical properties of polycaprolactone/collagen/elastin nanofibers fabricated by electrospinning

Abstract

Collagen and elastin are the two most abundant proteins in the human body, and as biomaterials offer fascinating properties to composite materials. More detailed investigations including these biomaterials within reinforced composites are still needed. This report describes physicochemical properties of fibers composed of collagen type I, collagen III, elastin and polycaprolactone (PCL). Prior to the electrospinning process, PCL was functionalized through covalent attachment of -NH₂ groups by aminolysis reaction with hexamentilendiamine. The fibers were fabricated by electrospinning technique set up with a non-conventional collector. A morphological comparative study was developed at different rations of collagen type I, observing in some cases two populations of fibers. The diameters and morphology were analyzed by SEM, observing a wide array of nanostructures with diameters of ~310 to 693nm. Chemical characterization was assessed by FT-IR spectroscopy and the functionalized PCL was characterized through ninhydrin assay resulting in 0.36mM NH₂/mg fiber. Swelling tests were performed for 24h, obtaining 320% for the majority of the fibers indicating morphological stability and good water uptake. In addition, contact angle analysis demonstrated adequate permeability and differences for each system depending mainly upon the type of biopolymer incorporated and the functionalization of PCL, ranging the values from 108° to 17°. Moreover, differential scanning calorimetry results showed a melting temperature (T_m) of ~60°C. The onset degradation temperatures (T_d,onset) ranged between 115 and 148°C, and were obtained by thermogravimetric analysis. The local mechanical properties of individual fibers were quantified by atomic force acoustic microscopy. These results propose that the physicochemical and mechanical properties of these scaffolds offer the possibility for enhanced biological activity Thus, they have a great potential as candidate scaffolds in tissue engineering applications.

Keywords: Collagen; Elastin; Electrospinning; Polycaprolactone.