Acclimation to NaCl and light stress of heterotrophic Chlamydomonas reinhardtii for lipid accumulation

Abstract

Salt stress has been proven very effective in enhancing the lipid content among many photoautotrophically grown microalgae species including marine and freshwater algae. Nevertheless, its effect on heterotrophic grown cells and lipid accumulation is scarcely known. This study sought to demonstrate a new train of thought for cost-effective biofuels production by heterotrophic culture of Chlamydomonas reinhardtii coupling with subsequent salt and light stress. NaCl treatments (25-200 mM) gradually suppressed the cell growth. After one day's acclimation, the cells restored slow growth with light supplement (200 μmol/m2/s) in low salt concentration (0-50 mM). However, high concentration of NaCl (200 mM) dose caused permanent damage, with over 47% cells death after 3 days treatment. The highest lipid content of 35.8% and lipid productivity of 28.6 mg/L/d were achieved by 50 mM NaCl stress and light treatment upon heterotrophic grown cells. Cells lost their green pigmentation and became yellowish under 100-200 mM NaCl conditions, whereas cells grown in 0-50 mM NaCl retained their dark-green pigmentation. Variable-to-maximum fluorescence ratio (Fv/Fm) and non-photochemical quenching (NPQ) value were markedly influenced under salt and light stress, indicating that severe inhibition of photosynthetic ability was occurred. Moreover, we further demonstrated the dynamic changes of cell growth and lipid accumulation would potentially be caused by the increase of intracellular redox state. To our knowledge, this study is the first instance in which C. reinhardtii was applied to oil accumulation by using combination of heterotrophic culture and multiple stress, and opened up a new territory for the further development of microalgae-based biofuels production.

Keywords: Chlamydomonas reinhardtii; Heterotrophy; Lipid; Physiological acclimation; Salt and light stress.