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Published Erratum
. 2017 May 16;114(20):E4112-E4114.
doi: 10.1073/pnas.1704696114. Epub 2017 May 8.

Correction for Dixon et al., Highly efficient delivery of functional cargoes by the synergistic effect of GAG binding motifs and cell-penetrating peptides

No authors listed
Published Erratum

Correction for Dixon et al., Highly efficient delivery of functional cargoes by the synergistic effect of GAG binding motifs and cell-penetrating peptides

No authors listed. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 2.
Fig. 2.
GET of Cre recombinase. (A) Schematic of the construct created to mark Cre activity in cells. Cre-mediated excision of a transcriptional STOP region flanked by loxP sites induces the constitutive expression of eGFP. Pr, promoter; βGal, β-galactosidase; Neo, Neomycin phosphotransferase. The NIH3t3 LSL-eGFP cell line was created by transfection and selection of NIH3t3 cells. (B) eGFP expression in untreated NIH3t3 LSP-eGFP cells or those transduced with SIN Cre lentivirus. (Left) Fluorescence microscopy. (Right) Flow cytometry histogram of eGFP expression. (Scale bar, 50 µm.) (C) Scheme of testing transduction of Cre activity in NIH3t3 LSL-eGFP cells. Cells were transduced with Cre proteins for 1 h and washed and cultured for 2 d before analyses. (D and E) P21-mR-Cre-8R is efficiently transduced and recombines DNA. (D) Fluorescence microscopy images Cre-transduced NIH3t3 LSL-eGFP with the variety of dosages. (Scale bar, 50 µm.) (E) Flow cytometry analyses of NIH3t3 LSL-eGFP cells transduced for 1 h with mR-Cre, mR-Cre-8R, and P21-mR-Cre-8R at a variety of dosages (0, 1, 10, 100, and 500 µg/mL), washed and cultured for 2 d. Graph shows percentage recombination (i.e., percentage of eGFP positive from total cell population). Error bars indicate SD. n = 6.
Fig. 3.
Fig. 3.
GET of NANOG promotes the self-renewal of mouse embryonic stem cells. (A) Scheme of testing activity of transduced NANOG in CGR-8 cells. Cells were transduced with P21-mR-NANOG-8R proteins (0, 1, 10, and 50 µg/mL) for 3 consecutive days (1 passage, 1:3 split), passaged 1:3, and plated into growth media with P21-mR-NANOG-8R but lacking LIF (−LIF). Cells were fed daily with –LIF media containing P21-mR-NANOG-8R and passaged 1:3 every 3 d for 2 passages (a total of 3 passages –LIF). (B) P21-mR-NANOG-8R rescues self-renewal of mESCs lacking LIF dose-dependently. AP staining of CGR-8 cells treated with P21-mR-NANOG-8R proteins and LIF withdrawal. AP activity and colony morphology is retained in CGR-8 cells cultured in LIF or without LIF but supplemented with SIN NANOG (to overexpress NANOG) or transduced with P21-mR-NANOG-8R. (Scale bar, 100 µm.) (C) P21-mR-NANOG-8R maintains the proliferation of mESCs lacking LIF dose-dependently. Percentage of the number of CGR-8 cells cultured without LIF versus those with LIF (percentage −LIF/+LIF) at passaging. In LIF-deficient CGR-8 cultures, proliferation is promoted when supplemented with SIN NANOG (to overexpress NANOG) or transduced with P21-mR-NANOG-8R. Error bars indicate SD. (D) NANOG-dependent rescue in LIF-deficient cultures generates a more epiblast-like gene expression profile. Relative gene expression analyses of LIF-deficient CGR-8 cultures using quantitative PCR. Cultures supplemented with SIN NANOG (to overexpress NANOG) or transduced with P21-mR-NANOG-8R have increased Fgf5 expression, reduced Rex1 expression, and retain Oct4 expression. Error bars indicate SE. n = 6.
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