Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017;58(2):86-95.
doi: 10.3358/shokueishi.58.86.

[Identification of Datura Species Involved in a Food-Poisoning Case Using LC-MS/MS and DNA Barcording]

[Article in Japanese]
Atsuko Ushiyama  1 , Chie Akaboshi  1 , Nobuhiko Ohsawa  1 , Tomomi Shimizu  1 , Yuki Matsushima  1 , Hideaki Shimizu  1 , Shigeki Hashiguchi  1
Affiliations
Free article

[Identification of Datura Species Involved in a Food-Poisoning Case Using LC-MS/MS and DNA Barcording]

[Article in Japanese]
Atsuko Ushiyama et al. Shokuhin Eiseigaku Zasshi. 2017.
Free article

Abstract

A food-poisoning case due to eating the roots of Datura occurred in Kawasaki City, Japan in 2014. The Datura plant was mistakenly collected instead of burdock in a domestic garden. The roots of these plants are quite similar to each other. We presumed that the specimen was the root of Datura, but it was difficult to classify it only from the morphology. Using LC-MS/MS, we detected atropine and scopolamine from the remaining plant specimen. Therefore, we applied the DNA barcoding method. The results showed that the specimen was classified into Solanaceae family, but not Asteraceae family. Thus, the specimen was confirmed to be Datura species based on both chemical and genetic analyses.

Keywords: Asteraceae family; Datura; LC-MS/MS; Solanaceae family; atropine; burdock; food poisoning; scopolamine; the DNA barcoding.

PubMed Disclaimer