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. 2017:2017:8015703.
doi: 10.1155/2017/8015703. Epub 2017 Apr 11.

Differential Expression of Serum Proteins in Rats with Allergic Asthma: A Study Based on the Nanoliter Two-Dimensional Liquid Chromatography Technique

Xingke Yan  1 , Ao Zhang  1 , Lu Yu  2 , Cheng Chen  3 , Haifu Cui  4
Affiliations

Differential Expression of Serum Proteins in Rats with Allergic Asthma: A Study Based on the Nanoliter Two-Dimensional Liquid Chromatography Technique

Xingke Yan et al. Can Respir J. 2017.

Abstract

Objective. To investigate the pathogenesis of allergic asthma via the nanoliter two-dimensional liquid chromatography (nano-2D-LC) technique. Method. 24 Wistar rats were randomized into 3 groups: the blank-control group (A), the restrained group (B), and the asthma model group (C). The nanoliter two-dimensional liquid chromatography (nano-2D-LC) technique was used to study the differential protein expressions of the serum in asthmatic rats. Results. Compared with the restrained group, the data from the asthma model group displayed a distinctive peak, that is, peak number 13 (94.731 min). The data also displayed three missing peaks in the asthma group, that is, peak number 1 (77.489 min), peak number 2 (78.418 min), and peak number 5 (80.533 min), suggesting that the corresponding peptides might be related to the pathogenesis of asthma. Compared with the blank-control group, the restrained group showed 4 new peaks, that is, peak number 2 (78.418 min), peak number 4 (79.398 min), peak number 5 (80.533 min), and peak number 7 (81.824 min). The restrained group also displayed a missing peak, that is, peak number 3 (78.804 min), indicating that those 5 polypeptides might be related to the binding-induced stress stimuli. Conclusion. The study suggests that the pathogenesis of allergic asthma is closely related to abnormal levels of proteins; however, future animal experiments should identify the specific protein expressions caused by stress factors.

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Figures

Figure 1
Figure 1
The peaks of peptides in the serum of 3 groups. (A) Blank-control group, (B) restrained group, and (C) asthma model group.
Figure 2
Figure 2
The peaks of peptides in the serum of blank-control group.
Figure 3
Figure 3
The peaks of peptides in the serum of restrained group.
Figure 4
Figure 4
The peaks of peptides in the serum of asthma model group.
See this image and copyright information in PMC

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