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. 2016 Aug 12;13(5):7-16.
doi: 10.21010/ajtcam.v13i5.2. eCollection 2016.

ANTIOXIDANT ACTIVITY AND FT-IR ANALYSIS OF DATURA INNOXIA AND DATURA METEL LEAF AND SEED METHANOLIC EXTRACTS

Khushboo Bhardwaj  1 , Sanjiv Kumar  1 , Sudarshan Ojha  1
Affiliations

ANTIOXIDANT ACTIVITY AND FT-IR ANALYSIS OF DATURA INNOXIA AND DATURA METEL LEAF AND SEED METHANOLIC EXTRACTS

Khushboo Bhardwaj et al. Afr J Tradit Complement Altern Med. .

Abstract

Background: Datura (family- Solanaceae), has a long history of being used as herbal medicine. These medicinal effects have been attributed to the phytochemicals present in the plant leaves and seeds, in particular alkaloids, flavonoids and phenolic compounds. The objective of this study was to investigate the methanolic leaf and seed extracts of Datura innoxia (DLP-I & DSP-I) and Datura metel (DLP-M & DSP-M) for their total phenolic, flavonoids and in-vitro antioxidant properties.

Materials and methods: Determination of total phenolic content and total flavonoid content and antioxidant activity in terms of total antioxidant assay, ABTS assay, DPPH assay and in-vitro lipid peroxidation inhibiting activity were determined along with the FT-IR (Fourier transform infrared spectroscopy) analysis of the extracts.

Results: The highest total phenolic and total flavonoid content were registered by the D. innoxia leaf extract (70.26 ±1.12 mg GAE/g and 34.24 ± 1.28 mg RE/g respectively). Maximum DPPH radical scavenging activity was exerted by the leaf extract of D. innoxia (IC50 = 146.69 ± 8.46 μg/mL) among the four different methanolic extracts. The highest activity of the ABTS assay was found in Datura innoxia leaf extract (IC50 value = 149.42 ± 13.43 μg/mL) and the highest total antioxidant capacity was found to be present in D. innoxia leaf extract (221.25 ± 1.06 mg AAE/g) whereas D. metel seed extract registered the maximum lipid peroxidation inhibition activity (IC50 = 112 ± 1.30 μg/mL). The FT-IR data also supported the maximum activity in D. innoxia (leaf and seed) extracts.

Conclusion: The results thus obtained suggested that the plant Datura innoxia possess considerable antioxidant activity over Datura metel and therefore can be established as a potential source of natural antioxidant.

Keywords: ABTS; Antioxidants; DPPH; Datura species; FT-IR; Methanolic extract.

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Conflict of interest statement

Conflict of interest The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
DPPH radical scavenging activity of Datura innoxia and Datura metel leaf & seed methanolic extracts. Values are represented as mean ± SD (n = 3). DLP-I (Datura innoxia leaf extract), DSP-I (Datura innoxia seeds extract), DLP-M (Datura metel leaf extract), DSP-M (Datura metel seeds extract).
Figure 2
Figure 2
ABTS radical cation scavenging activity of Datura innoxia and Datura metel leaf & seed methanolic extracts. Values are represented as mean ± SD (n = 3). DLP-I (Datura innoxia leaf extract), DSP-I (Datura innoxia seeds extract), DLP-M (Datura metel leaf extract), DSP-M (Datura metel seeds extract).
Figure 3
Figure 3
Lipid peroxidation inhibition activity of Datura innoxia and Datura metel leaf & seed methanolic extracts. Values are represented as mean ± SD (n = 3). DLP-I (Datura innoxia leaf extract), DSP-I (Datura innoxia seeds extract), DLP-M (Datura metel leaf extract), DSP-M (Datura metel seeds extract).
Figure 4
Figure 4
FT-IR spectra of methanolic extract of Datura innoxia leaves
Figure 5
Figure 5
FT-IR spectra of methanolic extract of Datura innoxia seeds
Figure 6
Figure 6
FT-IR spectra of methanolic extract of Datura metel leaves
Figure 7
Figure 7
FT-IR spectra of methanolic extract of Datura metel seeds
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