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Comparative Study
. 2017 May 10;22(5):771.
doi: 10.3390/molecules22050771.

Comparative Analysis of the Major Chemical Constituents in Salvia miltiorrhiza Roots, Stems, Leaves and Flowers during Different Growth Periods by UPLC-TQ-MS/MS and HPLC-ELSD Methods

Huiting Zeng  1   2 , Shulan Su  3 , Xiang Xiang  4 , Xiuxiu Sha  5 , Zhenhua Zhu  6 , Yanyan Wang  7 , Sheng Guo  8 , Hui Yan  9 , Dawei Qian  10 , Jinao Duan  11
Affiliations
Comparative Study

Comparative Analysis of the Major Chemical Constituents in Salvia miltiorrhiza Roots, Stems, Leaves and Flowers during Different Growth Periods by UPLC-TQ-MS/MS and HPLC-ELSD Methods

Huiting Zeng et al. Molecules. .

Abstract

Salvia miltiorrhiza is a traditional Chinese herbal medicine containing multiple components that contribute to its notable bioactivities. This article investigated the distribution and dynamic changes of chemical constituents in various parts of S. miltiorrhiza from different growth periods. An ultra-high performance liquid chromatography-triple quadrupole mass spectrometer (UPLC-TQ-MS/MS) and high-performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD) methods were developed for accurate determination of 24 compounds (including phenolic acids, flavonoids, triterpenes, and saccharides) in S. miltiorrhiza. The established methods were validated with good linearity, precision, repeatability, stability, and recovery. Results indicated that there were category and quantity discrepancies in different parts of the plant, for the roots mainly contained salvianolic acids and tanshinones, and most of the saccharides are stachyose. In the aerial parts, salvianolic acids, flavonoids, and triterpenes, except the tanshinones, were detected, and the saccharides were mainly monosaccharides. Dynamic accumulation analysis suggested the proper harvest time for S. miltiorrhiza Bunge was the seedling stage in spring, and for the aerial parts was July to August. This study provided valuable information for the development and utilization value of the aerial parts of S. miltiorrhiza and was useful for determining the optimal harvest time of the plant.

Keywords: S. miltiorrhiza Bunge; aerial parts; distribution; dynamic changes; harvest time.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Chemical structures of 24 reference compounds in S. miltiorrhiza.
Figure 2
Figure 2
UPLC-TQ-MS/MS chromatogram of 20 compounds in S. miltiorrhiza.
Figure 3
Figure 3
HPLC-ELSD chromatogram of different parts in S. miltiorrhiza. (A) Mixed reference; (B) roots; (C) stems; (D) leaves; and (E) flowers; (1) fructose; (2) glucose; (3) sucrose; (4); stachyose.
Figure 4
Figure 4
Dynamic accumulation of the contents of salvianolic acids and tanshinones in the roots of S. miltiorrhiza.
Figure 5
Figure 5
Dynamic accumulation of the contents of salvianolic acids, flavonoids, and triterpenes in the stems of S. miltiorrhiza.
Figure 6
Figure 6
Dynamic accumulation of the contents of salvianolic acids, flavonoids, and triterpenes in the leaves of S. miltiorrhiza.
Figure 7
Figure 7
Dynamic accumulation of the contents of salvianolic acids, flavonoids, and triterpenes in the flowers of S. miltiorrhiza.
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