Intracellular adenosine formation and its carrier-mediated release in cultured embryonic chick heart cells
- PMID: 2849008
- DOI: 10.1016/s0024-3205(88)80002-x
Intracellular adenosine formation and its carrier-mediated release in cultured embryonic chick heart cells
Abstract
Adenosine formation and release was examined in 48 hr old primary cultures of chick ventricular myocytes. Dilazep greater than hexobendine greater than dipyridamole inhibit incorporation of adenosine into chick embryonic heart cellular nucleotides in a concentration dependent manner. A combination of 30 mM 2-deoxyglucose and 2 micrograms of oligomycin/ml reduces the ATP content of the cells by 71% in 10 min. This change is accompanied by an increase in total adenosine concentration of 3.4 nmoles/10(7) cells in 10 min. Although the ATP concentration is not altered during hypoxia (95%N2/5%CO2), adenosine concentration increases by 0.52 nmoles/10(7) cells in 30 min. When nucleoside incorporation is inhibited by 85-90% by dipyridamole, dilazep or hexobendine, efflux of adenosine decreases by 70-90%, and 60-90% of the newly formed adenosine is trapped inside the cells compared to 10% in the absence of the transport inhibitors. alpha, beta -Methylene ADP inhibits the ecto 5'-nucleotidase activity by 91 +/- 6% but does not inhibit adenosine formation or alter its distribution between cells and medium, thus ruling out the involvement of this enzyme in adenosine formation. We conclude that adenosine is formed intracellularly during 2-deoxyglucose and oligomycin-induced ATP degradation and during hypoxia and that the nucleoside is released via the symmetric nucleoside transporter.
Similar articles
-
Effect of 5'-deoxy-5'-isobutylthioadenosine on formation and release of adenosine from neonatal and adult rat ventricular myocytes.Biochem J. 1993 May 1;291 ( Pt 3)(Pt 3):833-9. doi: 10.1042/bj2910833. Biochem J. 1993. PMID: 8489509 Free PMC article.
-
Adenosine formation and release by embryonic chick neurons and glia in cell culture.J Neurochem. 1989 Dec;53(6):1852-60. doi: 10.1111/j.1471-4159.1989.tb09252.x. J Neurochem. 1989. PMID: 2553868
-
Adenosine formation and release from neonatal-rat heart cells in culture.Biochem J. 1985 Aug 1;229(3):799-805. doi: 10.1042/bj2290799. Biochem J. 1985. PMID: 2996488 Free PMC article.
-
Purine metabolism in the heart. Strategies for protection against myocardial ischaemia.Pharm World Sci. 1994 Apr 15;16(2):69-76. doi: 10.1007/BF01880658. Pharm World Sci. 1994. PMID: 8032344 Review.
-
Regulation of adenosine formation by the heart.Circ Res. 1986 Feb;58(2):193-201. doi: 10.1161/01.res.58.2.193. Circ Res. 1986. PMID: 3004778 Review.
Cited by
-
Partial purification and properties of an AMP-specific soluble 5'-nucleotidase from pigeon heart.Biochem J. 1990 May 15;268(1):117-22. doi: 10.1042/bj2680117. Biochem J. 1990. PMID: 2344353 Free PMC article.
-
Adenine nucleotide degradation in cultured chick heart muscle cells.Mol Cell Biochem. 1991 Oct 16;107(2):119-125. doi: 10.1007/BF00225515. Mol Cell Biochem. 1991. PMID: 1791825
-
The influence of calcium antagonists on the adenine nucleotide metabolism in the guinea-pig working heart during ischaemia and reperfusion.Naunyn Schmiedebergs Arch Pharmacol. 1991 May;343(5):496-504. doi: 10.1007/BF00169552. Naunyn Schmiedebergs Arch Pharmacol. 1991. PMID: 1881460
-
Effect of 5'-deoxy-5'-isobutylthioadenosine on formation and release of adenosine from neonatal and adult rat ventricular myocytes.Biochem J. 1993 May 1;291 ( Pt 3)(Pt 3):833-9. doi: 10.1042/bj2910833. Biochem J. 1993. PMID: 8489509 Free PMC article.
-
A hypoxic episode during cardiogenesis downregulates the adenosinergic system and alters the myocardial anoxic tolerance.Am J Physiol Regul Integr Comp Physiol. 2015 Apr 1;308(7):R614-26. doi: 10.1152/ajpregu.00423.2014. Epub 2015 Jan 28. Am J Physiol Regul Integr Comp Physiol. 2015. PMID: 25632022 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Research Materials