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. 2017 Dec;10(1):18.
doi: 10.1186/s12284-017-0159-0. Epub 2017 May 11.

Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast

Chaivarakun Chaipanya  1   2 , Mary Jeanie Telebanco-Yanoria  2 , Berlaine Quime  2 , Apinya Longya  1 , Siripar Korinsak  3 , Siriporn Korinsak  3 , Theerayut Toojinda  4 , Apichart Vanavichit  5   6 , Chatchawan Jantasuriyarat  7   8 , Bo Zhou  9
Affiliations

Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast

Chaivarakun Chaipanya et al. Rice (N Y). 2017 Dec.

Abstract

Background: Rice (Oryza sativa) is one of the most important food crops in the world. Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most destructive rice diseases worldwide. To effectively cope with this problem, the use of rice blast resistance varieties through innovative breeding programs is the best strategy to date. The Thai rice variety Jao Hom Nin (JHN) showed broad-spectrum resistance against Thai rice blast isolates. Two QTLs for blast resistance in JHN were reported on chromosome 1 (QTL1) and 11 (QTL11).

Results: Monogenic lines of QTL1 (QTL1-C) and QTL11 (QTL11-C) in the CO39 genetic background were generated. Cluster analysis based on the disease reaction pattern of QTL1-C and QTL11-C, together with IRBLs, showed that those two monogenic lines were clustered with IRBLsh-S (Pish) and IRBL7-M (Pi7), respectively. Moreover, sequence analysis revealed that Pish and Pi7 were embedded within the QTL1 and QTL11 delimited genomic intervals, respectively. This study thus concluded that QTL1 and QTL11 could encode alleles of Pish and Pi7, designated as Pish-J and Pi7-J, respectively. To validate this hypothesis, the genomic regions of Pish-J and Pi7-J were cloned and sequenced. Protein sequence comparison revealed that Pish-J and Pi7-J were identical to Pish and Pi7, respectively. The holistic disease spectrum of JHN was found to be exactly attributed to the additive ones of both QTL1-C and QTL11-C.

Conclusion: JHN showed broad spectrum resistance against Thai and Philippine rice blast isolates. As this study demonstrated, the combination of two resistance genes, Pish-J and Pi7-J, in JHN, with each controlling broad-spectrum resistance to rice blast disease, explains the high level of resistance. Thus, the combination of Pish and Pi7 can provide a practical scheme for breeding durable resistance in rice against rice blast disease.

Keywords: Durable resistance; Magnaporthe oryzae; Quantitative trait loci (QTL).

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Figures

Fig. 1
Fig. 1
The resistance frequency of IRBLs and JHN against Philippine rice blast isolates. The resistance frequency of each rice variety was calculated based on the resistance reactions against 132 Philippine isolates as listed in Additional file 1: Table S1. The resistance frequency of JHN was indicated on the top of the bar
Fig. 2
Fig. 2
An integrated genetic and physical map of QTL1 (a) and QTL2 (b). The chromosomal position of SSR markers delimiting the genomic interval of QTLs by referring to the one in the reference genome of Nipponbare was indicated below the line. The number of recombinants/toal F3 progeny at each SSR marker was indicated above the line. Four and 9 NBS-LRR type R genes predicted in the genomic intervals in Nipponbare for QTL1 and QTL11, respectively, were indicated in boxes with gene IDs. The figure was not draw in scale
Fig. 3
Fig. 3
Cluster analysis of disease reaction patterns of QTL1-C, QTL11-C, and IRBLs against 42 representative blast isolates. The lines of QTL1-C and QTL11-C are highlighted in asterisks
See this image and copyright information in PMC

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