Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Apr 20;25(4):273-278.
doi: 10.3760/cma.j.issn.1007-3418.2017.04.008.

[Regulatory effect of bone marrow mesenchymal stem cells on polarization of macrophages]

[Article in Chinese]
Y Hou  1 , X Zhou  1 , W L Cai  1 , C C Guo  2 , Y Han  2
Affiliations

[Regulatory effect of bone marrow mesenchymal stem cells on polarization of macrophages]

[Article in Chinese]
Y Hou et al. Zhonghua Gan Zang Bing Za Zhi. .

Abstract
in English, Chinese

Objective: To examine the regulatory effect of bone marrow mesenchymal stem cells (BM-MSCs) on the polarization of bone marrow-derived macrophages, and to provide a theoretical support for the application of mesenchymal stem cells in the treatment of liver fibrosis. Methods: MSCs and macrophages were first isolated from the bone marrow of mice. Macrophages were polarized to M1 macrophages with lipopolysaccharide (LPS) and interferon-γ (IFN-γ), and to M2 macrophages with interleukin-4 (IL-4). The macrophages were then co-cultured with BM-MSCs in a Transwell for 24 h, and changes in the percentages of M1 and M2 macrophages were examined using flow cytometry. The mRNA levels of the M1 macrophage-associated cytokines, tumor necrosis factor-α (TNF-α) and interleukin-23a (IL-23a), and M2 macrophage-associated molecules, arginase-1 (Arg-1) and CD163, were measured by real-time quantitative PCR. The two samples were compared using the t test, and P < 0.05 was considered as statistically significant. Results: Flow cytometry showed that the percentage of M1 macrophages was significantly lower in the (macrophage + LPS + IFN-γ + BM-MSC) co-culture group than in the (macrophage + LPS + IFN-γ) group (62.5% ± 4.6% vs 86.6% ± 6.9%, t = 5.034, P = 0.0073). In addition, the relative mRNA expression of TNF-α and IL-23a was also significantly reduced in the co-culture group compared with those in the macrophage control group as measured by RT-qPCR (t = 11.57 and 10.57, respectively, P < 0.05). Compared with that in the macrophage control group, the percentage of M2 macrophages in the (macrophage+BM-MSC) co-culture group was significantly increased (89.5% ± 5.8% vs 70.1% ± 6.3%, t = 3.924, P = 0.0172), along with significantly elevated relative mRNA expression of Arg1 (14.35±1.05 vs 1.00±0.03, t = 21.96, P < 0.05) and CD163 (3.04 ± 0.27 vs 1.00 ± 0.03, t = 13.14, P < 0.05). Conclusion: BM-MSCs can inhibit LPS + IFN-γ-induced polarization to M1 macrophages and promote polarization to M2 macrophages through the release of paracrine factors.

目的： 探索骨髓间充质干细胞（BM-MSC）对骨髓来源巨噬细胞极化的影响，为间充质干细胞治疗肝纤维化提供理论依据。 方法： 分别分离小鼠BM-MSC和骨髓来源巨噬细胞，用脂多糖（LPS）和干扰素γ（IFNγ）或白细胞介素4（IL-4）刺激巨噬细胞向M1或M2型巨噬细胞极化。利用Transwell小室建立BM-MSC和巨噬细胞非接触式共培养体系，通过流式细胞术分析共培养后，M1型巨噬细胞和M2型巨噬细胞比例变化情况，通过荧光定量PCR检测M1型巨噬细胞相关分子肿瘤坏死因子α（TNFα）、白细胞介素23a（IL-23a）和M2型巨噬细胞相关分子精氨酸酶1（Arg1）、CD163表达量的变化。两样本比较采用t检验，P<0.05为差异有统计学意义。 结果： 巨噬细胞+ LPS + IFNγ+ BM-MSC共培养组与巨噬细胞+ LPS+IFNγ组比较，M1型巨噬细胞比例显著降低（62.5%±4.6%对比86.6%±6.9%，t = 5.034，P = 0.0073），差异有统计学意义；且M1型巨噬细胞相关分子TNFα相对表达量（3.75±0.23对比6.17±0.27）和IL-23a相对表达量（2.05±0.16对比4.56±0.37），均明显降低，t值分别为11.57、10.57，P值均< 0.05，差异均具有统计学意义。巨噬细胞+ BM-MSC共培养组与巨噬细胞单独培养组相比，M2型巨噬细胞比例显著增高（89.5%±5.8%对比70.1%±6.3%，t = 3.924，P = 0.0172），差异有统计学意义；且M2型巨噬细胞相关分子Arg1相对表达量（14.35±1.05对比1.00±0.03）和CD163相对表达量（3.04±0.27对比1.00±0.03）均显著增高，t值分别为21.96、13.14，P值均< 0.05，差异均具有统计学意义。 结论： BM-MSC可通过旁分泌作用抑制巨噬细胞由LPS + IFNγ诱导的M1型极化，促进巨噬细胞向M2型极化。.

Keywords: Bone marrow mesenchymal stem cells; Liver cirrhosis; Macrophage polarization.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles